生物产业技术Issue(1)：76-82,7.DOI:10.3969/j.issn.1674-0319.2017.01.012
生物质降解酶生产菌株的育种和应用
Screening of cellulase hyper-producing strains and their application
摘要
Abstract
Although China becomes the world’s second largest economy by nominal GDP, there are many problems, such as energy crisis, resource exhaustion and environmental pollution. Because the depletion of fossil fuels in the future and bioethanol is a renewable and carbon neutral alternative liquid fuel, bioethanol production from biomass was being supported by the Chinese government. <br> Enzymatic hydrolysis for obtaining the fermentable sugar is a key method in bioethanol production process from biomass. Furthermore, screening strains with cellulase hypersecreting ability is a core technology that has a significant effect on production cost of biomass biorefinery. In this paper, we introduce the industrial microorganisms producing the cellulolytic enzyme, the physical and chemical mutagens, and genetic reconstruction of the industrial strains. Two successful cases of biomass biorefinery with cellulolytic enzyme are also stated.关键词
生物乙醇/纤维素酶/微生物育种/酶水解Key words
bioethanol/cellulase/microbial screening/enzymatic hydrolysis引用本文复制引用
王方忠,张睿钦,金建玲,夏蕊蕊,肖林,易勇,张继祥,侯少莉,方诩..生物质降解酶生产菌株的育种和应用[J].生物产业技术,2017,(1):76-82,7.基金项目
山东省自主创新及专项成果转化项目（2014CGZH1312），国家高技术研究发展计划“863计划”（2014AA021903），国家自然科学基金（31570040），山东大学基本科研业务费资助项目-自然科学专项（交叉学科培育项目）（2016JC031），中国博士后面上基金 （）
