生物加工过程2017，Vol.15Issue(1)：37-42,6.DOI:10.3969/j.issn.1672-3678.2017.01.006
高聚物型色谱柱在高效液相色谱法中一步分析制备葛根素及大豆苷元的应用
One-step determination and preparation of puerarin and daidzein by polymer column in HPLC
摘要
Abstract
Polymeric chromatographic column with polystyrene⁃divinyl⁃benzene ( PS⁃DVB ) packing materials ( MKF⁃RP⁃HH,300 mm×7�8 mm,8 μm) was established to determinate and prepare puerarin and daidzein.The kudzu extraction dissolved and diluted by 70% ethanol.Water (A)/methanol (B) was served as mobile phase at the rate of 1�0 mL/min.Detection was done at the wavelength of 250 nm at 30℃.Two active ingredients ( puerarin and daidzein) and impurities have achieved effective separation. Column efficiency reached 2 100 N/m that is superior than conventional silica column (1 000 N/m).This polymeric column can be used as semi⁃preparative column to prepare puerarin and daidzein.The selected chromatographic conditions led to effectively determinating puerarin with good linearity within 2⁃50μg/mL,the regression equation was y ( puerarin) =1�342 2x-1�018 4,and the regression coefficient was 0�999;the selected chromatographic conditions were found to effectively determinate daidzein with good linearity within 2⁃20 μg/mL, the regression equation was y ( daidzein )= 2�275x+0�869 5, and the regression coefficient was 0�999.The purification efficiency of puerain and daidzein could reach to 95�9%and 78�5%.关键词
聚苯乙烯/二乙烯基苯/高聚物型色谱柱/反相高效液相色谱法/葛根素/大豆苷元Key words
polystyrene-divinyl-benzene/polymeric chromatographic column/reversed phase high-performance liquid chromatography/puerarin/daidzein分类
化学化工引用本文复制引用
夏明,刘晓宁,魏荣卿,郑涛..高聚物型色谱柱在高效液相色谱法中一步分析制备葛根素及大豆苷元的应用[J].生物加工过程,2017,15(1):37-42,6.基金项目
国家重点基础研究发展计划（973计划）（2013CB733504）；国家高技术研究发展计划（863计划） （）
