生物加工过程2017，Vol.15Issue(1)：69-72,4.DOI:10.3969/j.issn.1672-3678.2017.01.011
兽药残留检测中磺胺嘧啶ELISA试剂盒的研制
Determination of sulfadiazine in the veterinary drugs residue by sensitive ELISA
摘要
Abstract
Using indirect competitive enzyme linked immunosorbent assay ( ELISA ) , a rapid, highly selective and extremely sensitive method has been established for the determination of sulfadiazine ( SD) . The SD detection limit was 0�213 ng/mL for indirect competitive ELISA formats,the assay ranges from 1�84 to 57�41 ng/mL,except for the cross reactivity with sulfadimidine is over 10%, cross reaction with other sulfadrugs were low than 1�5%, and there was no significant change under 4 ℃ in 180 d. The within⁃run and between⁃run CVs of the SD⁃ELISA were less than 10% and 15%,respectively. The mean recoveries from the SD⁃free pig urine, milk and pork were 75% to 90%. The results show that the SD⁃ELISA method can be applied to detect the SD contamination in food and animal feed.关键词
磺胺嘧啶/间接竞争ELISA/回收实验/交叉反应率Key words
sulfadiazine/indirect competitve ELISA/recovery test/cross-reaction rate分类
轻工纺织引用本文复制引用
田博,金坚,惠人杰,徐凤莲,吴涛,李伟..兽药残留检测中磺胺嘧啶ELISA试剂盒的研制[J].生物加工过程,2017,15(1):69-72,4.基金项目
江苏省自然科学基金青年项目 （）
