生物技术通报2017，Vol.33Issue(1)：148-156,9.DOI:10.13560/j.cnki.biotech.bull.1985.2017.01.017
谷氨酸棒状杆菌合成5-氨基乙酰丙酸的途径构建与发酵优化
Construction of 5-aminolevulinic Acid Synthesis Pathway and Optimization of Fermentation by Corynebacterium glutamicum
摘要
Abstract
5-aminolevulinic acid(ALA)is a natural non-protein amino acid and has been widely applied in agriculture and medicine. This study aimed to construct the systhesis pathway of C4 ALA in Corynebacterium glutamicum and optimize its fermentation process. First, efficient C4 ALA synthesis pathway was established in C. glutamicum by over-expressing the ALA synthase(ALAS)from Rhodopseudomonas palustris. Then,the ALA fermentation process with flask was optimized from four factors :fermentation medium,concentration of inducer, substrate concentration,and initial inoculum dosage. As results,the ALA yield of the strain 13032/pZWA1 over-expressing HemA was 1.41 g/L,up to 67.14 folds compared with the control strain. The optimal ALA fermentation condition was M9 media using yeast extract as nitrogen source with 5% inoculum size,0.1 mmol/L IPTG to induce the experession of HemA,and the concentration of glycine must be at 4 g/L. After optimization,ALA yield reached 3.28 g/L in a shaking flask and increased 132.62% than before. In conclusion,under the optimal fermentation condition,the ALA yield in a 5 L bioreactor fermentation was 10.08 g/L,which was the highest ALA yield by one-step fermentation of C. glutamicum reported so far.关键词
5-氨基乙酰丙酸/谷氨酸棒状杆菌/5-氨基乙酰丙酸合成酶/沼泽红假单胞菌/发酵优化Key words
5-aminolevulinic acid/Corynebacterium glutamicum/5-aminolevulinic acid synthetase/Rhodopseudomonas palustris ATCC17001/optimization of fermentation引用本文复制引用
饶德明,张良程,陈久洲,孙德虎,孙村民,郑小梅,郑平,刁爱坡..谷氨酸棒状杆菌合成5-氨基乙酰丙酸的途径构建与发酵优化[J].生物技术通报,2017,33(1):148-156,9.基金项目
天津市科技支撑计划重点项目 （）
