西安交通大学学报（医学版）2017，Vol.38Issue(1)：18-23,28,7.DOI:10.7652/jdyxb201701004
ERK信号通路抑制剂U0126对蛛网膜下腔出血大鼠早期脑损伤及神经元自噬的影响
The influence of U0126 of ERK signaling pathway inhibitor on early brain inj ury and neurons autophagy in SAH rats
摘要
Abstract
ABSTRACT:Objective To explore role of U0126,the specific inhibitor of ERK signaling pathway,in early brain injury (EBI)and the autophagy of nerve cells in hippocampus area in subarachnoid hemorrhage (SAH). Methods A total of 48 male adult SD rats were randomly divided into control group,SAH group,DMSO+SAH group,and U0126+SAH group,with 12 in each.We established SAH rat model by the puncture of internal carotid artery.The same amount of saline water,DMSO and U0126 solution of 0.5 mL per rat was injected respectively into the rats of different groups 30 min before modeling.The rats were killed at 24 h.To measure brain water content by Wet and dry method after 24 h,the morphological changes of hippocampus CA1 neural cells were observed by microscopy;the expression levels of ERK,Beclin-1 and LC3 were detected by using immunohistochemical method. Results Compared with that in sham group,brain water content increased obviously in SAH model group.The density of surviving neurons in SAH group was significantly lower than that in control group (P<0 .0 5 ).ERK signaling pathway was activated obviously,the expressions of Beclin 1 and LC3-Ⅱ were significantly higher than those in control group (P<0.05).Compared with SAH model group,in U0126 group brain water content increased obviously.Compared with those in SAH group,the density of surviving neurons was significantly lower (P<0.05), ERK signaling pathway was suppressed,the expressions of Beclin-1 and LC3-Ⅱ were significantly lower (P<0.05). Conclusion The U0126,the ERK signaling pathway inhibitor,can inhibit neuron autophagy and increase EBR of SAH.关键词
蛛网膜下腔出血/早期脑损伤/自噬/ERKKey words
subarachniod hemorrhage/early brain injury/autophagy/extracellular regulated protein kinase (ERK)分类
医药卫生引用本文复制引用
刘俊杰,赵雅宁,陈禹廷,付程凯,丁家杉,徐继伟,李建民..ERK信号通路抑制剂U0126对蛛网膜下腔出血大鼠早期脑损伤及神经元自噬的影响[J].西安交通大学学报（医学版）,2017,38(1):18-23,28,7.基金项目
河北省重大医学课题(No.ZD2013093) （No.ZD2013093）
唐山市科技计划项目(No.14130220B) Supported by the Major Medical Projects of Hebei Province (No.ZD2013093)and Science and Technology Plan Projects of Tangshan (No.14130220B) （No.14130220B）
