西安交通大学学报（医学版）2017，Vol.38Issue(1)：24-28,5.DOI:10.7652/jdyxb201701005
Ac-SDKP调控Gαs/Gαi信号抑制大鼠矽肺纤维化的作用
Ac-SDKP inhibits silicosis fibrosis in rats by regulating Gαs/Gαi signaling
摘要
Abstract
ABSTRACT:Objective To observe whether N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP)can inhibit rat silicotic fibrosis by regulating stimulatory G proteinα(Gαs)/inhibitory G proteinα(Gαi)signal.Methods Male Wistar rats were randomly divided into three groups (n=1 0 ):control 1 6-w group,silicosis 1 6-w group,and Ac-SDKP pre-treatment group.The pathological changes of the lung tissue was observed by HE staining;the expressions ofα-smooth muscle actin (α-SMA),Collagen Ⅰ,fibronectin (Fn),Gαs,Gαi2 ,Gαi3 and cAMP were detected by Western blot.Immunofluorescence was performed on lung tissue sections to detect the coexpression ofα-SMA/Gαi3 . Results Within silicosis 16-w group,HE staining showed that the silicotic nodule volume increased,nodule fusion and the formation of interstitial fibrosis could be seen,and cell fibrous nodules were visible.Immunofluorescence staining showed the enhanced coexpression ofα-SMA/Gαi3 in fibrosis area.Compared with those in control group, the expressions ofα-SMA,Collagen Ⅰ,Fn,Gαi2 and Gαi3 significantly increased in silicosis 1 6-w group,but the expressions of Gαs and cAMP decreased.Compared with silicosis 1 6-w group,Ac-SDKP pre-treatment group had alleviated lung injury and decreased coexpression ofα-SMA/Gαi3 .The expressions ofα-SMA,Collagen Ⅰ,Fn,Gαi2 and Gαi3 protein significantly decreased in Ac-SDKP pre-treatment group,while the expressions of Gαs and cAMP increased obviously.Conclusion Ac-SDKP can regulate the expressions of Gαs and Gαi and promote the formation of cAMP,thus playing an effective role against silicotic fibrosis.关键词
N-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸/矽肺病/环磷酸腺苷/激动型 G蛋白α/抑制性 G蛋白αKey words
N-acetyl-seryl-aspartyl-lysyl-proline/silicosis/cyclic adenosine monophosphate/Gαs/Gαi分类
医药卫生引用本文复制引用
刘燕,耿玉聪,徐洪,张丽娟,王建辉,王瑞雪,胡启峰,杨方..Ac-SDKP调控Gαs/Gαi信号抑制大鼠矽肺纤维化的作用[J].西安交通大学学报（医学版）,2017,38(1):24-28,5.基金项目
国家自然科学基金资助项目(No.81472953),河北省自然科学基金青年项目(No.H2016209161) Supported by the National Natural Science Foundation of China (No.81472953)and the Natural Science Foundation of Hebei Province (No.H2016209161) （No.81472953）
