西安交通大学学报（医学版）2017，Vol.38Issue(1)：29-33,57,6.DOI:10.7652/jdyxb201701006
糖尿病心肌缺血大鼠骨髓干细胞动员及心肌组织细胞因子的表达
Bone marrow stem cell mobilization and cytokines expression in ischemic myocardium in diabetic rats with myocardial ischemia
摘要
Abstract
ABSTRACT:Objective To investigate the bone marrow stem cell mobilization and homing changes of peripheral blood due to diabetic myocardial ischemia in rats and the change of expression of the vascular endothelial growth factor (VEGF)and the tumor necrosis factorα(TNFα)in ischemic myocardial tissue.Methods The diabetic model of male Sprague-Dawley rats was created by intraperitoneal injection of streptozotocin first.Then the diabetic myocardial ischemia model was established through ligation of the left anterior descending coronary artery. The rats were divided into ischemic day-1,day-3,day-7,and day-28 groups.And their time-matching diabetic sham operation control group and normal sham operation control group were also established,with 6 rats in each group. Flow cytometry was used to measure the percentage of CD34+ cells in peripheral blood mononuclear cells for each group.Immunohistochemistry was used to observe the homing of CD34+ cells and the expression of VEGF and TNFαin the ischemic myocardium.Results The mobilization of bone marrow stem cells was initiated after the surgery in the rat models of myocardial ischemia in diabetes and it peaked within 1 week and decreased with the passage of time.Accordingly the homing of CD34+ cells and the expressions of VEGF and TNFαin the ischemic myocardium were significantly increased.Conclusion During the early stage,the diabetic ischemic myocardium can mobilize bone marrow stem cells into the peripheral blood and start homing to the ischemic myocardium by increasing VEGF and TNFαin the ischemic myocardium,thereby initiating the self-protection mechanisms.关键词
心肌缺血/糖尿病/骨髓干细胞/血管内皮生长因子/肿瘤坏死因子αKey words
myocardial ischemia/diabetes/bone marrow stem cell/vascular endothelial growth factor (VEGF)/tumor necrosis factorα(TNFα)分类
医药卫生引用本文复制引用
赵庆斌,刘丹丹,周娟..糖尿病心肌缺血大鼠骨髓干细胞动员及心肌组织细胞因子的表达[J].西安交通大学学报（医学版）,2017,38(1):29-33,57,6.基金项目
国家自然科学基金资助项目(No.81200098) （No.81200098）
陕西省自然科学基金项目(No.2014K11-03-04-06) Supported by the National Natural Science Foundation of China (No.81200098)and the Natural Science Foundation of Shaanxi Province (No.2014K11-03-04-06) （No.2014K11-03-04-06）
