西安交通大学学报（医学版）2017，Vol.38Issue(1)：34-38,43,6.DOI:10.7652/jdyxb201701007
SDF-1/CXCR7对胃癌SGC-7901细胞合成及分泌炎症因子的影响
Effect of SDF-1/CXCR7 on inflammatory cytokine synthesis and secretion in gastric cancer SGC-7901 cells
摘要
Abstract
ABSTRACT:Objective To investigate the effect of SDF-1/CXCR7 on inflammatory cytokine synthesis and secretion in gastric cancer SGC-7901 cells.Methods CXCR7 gene in SGC-7901 cells was silenced by shRNA lentiviral vector and the expression of CXCR7 was detected using Western blot and Real-time PCR.There were four groups as follows:LV-shRNA-NC,LV-shRNA-NC+SDF-1,LV-shRNA-CXCR7,and LV-shRNA-CXCR7+SDF-1 groups.Real-time PCR was used to detect the mRNA expressions of TNF-α,IL-1β,IL-6 and IL-8.ELISA was used to detect the protein levels of TNF-α,IL-1β,IL-6 and IL-8 in the culture supernatant.Western blot was used to detect the protein expressions of NF-κB pathway.Results ① Transfection of SGC-7901 cells with CXCR7-shRNA lentiviral vector resulted in a significantly decreased expression of CXCR7 at both mRNA and protein levels (all P<0.01).② Compared with those in LV-shRNA-NC group,IL-6 and IL-8 mRNA expressions and protein levels in the culture supernatant were increased in LV-shRNA-NC+SDF-1 group (P<0.01 )and decreased in LV-shRNA-CXCR7 group (P<0.05).Compared with those in LV-shRNA-NC+SDF-1 group,the expressions of IL-6 and IL-8 at mRNA and protein levels in the culture supernatant were significantly cut down in LV-shRNA-CXCR7+SDF-1 group (P<0.01 ).However,the expressions of TNF-αand IL-1βat mRNA and protein levels in the culture supernatant were not significantly changed by SDF-1 and CXCR7 shRNA.③ The protein expressions of nuclear NF-κB p65,t-IκBαand p-IκBαexhibited no significant differences among the four groups.Conclusion SDF-1/CXCR7 can promote the synthesis and secretion of inflammatory cytokines IL-6 and IL-8 in gastric cancer SGC-7 9 0 1 cells through an NF-κB-independent pathway.关键词
胃癌/CXC趋化因子受体 7/基质细胞衍生因子-1 (SDF-1 )/炎症因子/NF-κBKey words
gastric cancer/CXCR7/SDF-1/inflammatory cytokine/NF-κB分类
医药卫生引用本文复制引用
师阿盟,史海涛,董蕾,许绍娴,郭晓燕,王婷..SDF-1/CXCR7对胃癌SGC-7901细胞合成及分泌炎症因子的影响[J].西安交通大学学报（医学版）,2017,38(1):34-38,43,6.基金项目
国家自然科学基金资助项目(No.81200310),国家星火计划项目(No.2011GAB50001),西安交通大学第二附属医院院基金[No.YJ (QN)201514] Supported by the National Natural Science Foundation of China (No.81200310),the National Spark Program (No.2011GAB50001), and the Foundation of the Second Affiliated Hospital of Xi’an Jiaotong University [No.YJ(QN)201514] （No.81200310）
