西安交通大学学报（医学版）2017，Vol.38Issue(1)：44-47,57,5.DOI:10.7652/jdyxb201701009
重组人松弛素-2对肝星状细胞增殖、Ⅰ型胶原合成及TGF-β1与CTGF mRNA表达的影响
Effects of recombinant human relaxin-2 on hepatic stellate cell proliferation, synthesis of Collagen Ⅰ,and mRNA expressions of TGF-β1 and CTGF in vitro
陈振 1许君望 1严肃 1陈珂 1范修德1
作者信息
- 1. 西安交通大学第一附属医院消化内科，陕西西安 710061
- 折叠
摘要
Abstract
ABSTRACT:Objective To validate that relaxin can resist hepatic fibrosis at the cellular level and explore its molecular mechanism in order to provide experimental basis for the treatment of liver cirrhosis.Methods Cultured HSC-T6s were treated with different concentrations (20,50 and 100 ng/mL)of recombinant human relaxin-2 (RLX-2).The proliferation of HSC-T6 was measured by MTT colorimetric assay.The content of type Ⅰcollagen in the cell culture supernatant of each group was detected by ELISA at 48 h of drug intervention;RT-PCR was used to detect the mRNA expressions of CTGF and TGF-β1 in HSC-T6 at 48 h of drug intervention.Results RLX-2 inhibited the proliferation of HSC and reduced type Ⅰ collagen content of HSC cells.It also inhibited the CTGF mRNA expression of HSC,but did not have a significant effect on the expression of TGF-β1 mRNA. Conclusion In the experiment of culturing HSC-T6 in vitro,RLX-2 may play a role in rat liver fibrosis by inhibiting cell proliferation and type Ⅰ collagen and CTGF mRNA expressions.关键词
肝纤维化/肝星状细胞/结缔组织生长因子(CTGF)/转化生长因子(TGF)/松弛素Key words
hepatic fibrosis/hepatic stellate cell/connective tissue growth factor (CTGF)/transforming growth factor(TGF)/relaxin分类
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陈振,许君望,严肃,陈珂,范修德..重组人松弛素-2对肝星状细胞增殖、Ⅰ型胶原合成及TGF-β1与CTGF mRNA表达的影响[J].西安交通大学学报（医学版）,2017,38(1):44-47,57,5.
