西安交通大学学报（医学版）2017，Vol.38Issue(1)：66-70,5.DOI:10.7652/jdyxb201701014
巨噬细胞在I gA肾病患者肾脏中的浸润及其与肾纤维化的关系
Macrophage infiltration in the kidney of patients with IgA nephropathy and its relationship with renal fibrosis
摘要
Abstract
ABSTRACT:Objective To analyze macrophage infiltration in the kidney of patients with IgA nephropathy and its relationship with renal fibrosis so as to explore the role of macrophages in the development of IgA nephropathy. Methods The kidney specimens from 40 patients with IgA nephropathy were obtained by diagnostic renal biopsy. The normal kidney specimens were obtained from 1 1 patients with nephrectomy after renal trauma.The specimens were treated by paraffin embedding and sliced for immunohistochemical staining with CD68 antibody which labelled macrophages.The level of CD68 positive staining in different grades of IgA nephropathy was analyzed by calculating staining area in randomly selected visual fields.Masson staining was used to evaluate the severity of renal fibrosis;the relationship between renal fibrosis and macrophage infiltration was analyzed. Results The level of macrophage infiltration in the kidney of patients with IgA nephropathy increased with the grade of IgA nephropathy.The ratios of the area in infiltrated macrophages to the area of kidney from grade Ⅱ to grade Ⅴ were 0.153% (0.058%),0.211% (0.101%),0.324% (0.132%)and 0.533% (0.071%);the ratio in normal kidney was 0.043% (0.04%).There were significant differences between the groups (P<0.01 ).There was a positive correlation between renal fibrosis and macrophage infiltration with the correlation coefficient of r=0 .9 0 1 (P<0.01).Conclusion Renal macrophage infiltration becomes more severe with the increased grade of IgA nephropathy,and it is positively correlated with renal fibrosis.It is indicated that macrophages are involved in the development of IgA nephropathy.关键词
巨噬细胞/IgA肾病/肾纤维化Key words
macrophage/IgA nephropathy/renal fibrosis分类
医药卫生引用本文复制引用
程小莉,姚钢炼,王莉,吕治安,杨艳艳,景兰梅,陈镝,赵玉峰,田李芳,付荣国,桂保松..巨噬细胞在I gA肾病患者肾脏中的浸润及其与肾纤维化的关系[J].西安交通大学学报（医学版）,2017,38(1):66-70,5.基金项目
国家自然科学基金资助项目(No.81300581、81470968、81400740) （No.81300581、81470968、81400740）
陕西省自然科学基金资助项目(No.2013JQ4024) Supported by the National Natural Science Foundation of China (No.81300581,81470968,and 81400740)and the Natural Science Foundation of Shaanxi Province (No.2013JQ4024) （No.2013JQ4024）
