西安交通大学学报（医学版）2017，Vol.38Issue(1)：113-116,4.DOI:10.7652/jdyxb201701024
腹腔镜手术时间延长对腹膜间皮细胞及纤维蛋白溶解活性的影响
The effect of prolonged laparoscopic surgery on peritoneal mesothelial cells and fibrinolysis
赵伟 1纪明明 2李晓婷 2李云东 2隋淑凤 2谭春林 2刘在波 2刘莉 2王学萍 2王康1
作者信息
- 1. 西安交通大学第一附属医院普外科，陕西西安 710061
- 2. 海阳市人民医院，山东海阳 265100
- 折叠
摘要
Abstract
ABSTRACT:Objective To assess the effect of prolonged laparoscopic surgery on peritoneal mesothelial cells and fibrinolysis in humans.Methods We examined prospectively 1 6 consecutive patients who underwent laparoscopic surgery (LAP)and 2 1 patients who underwent conventional open surgery (OP)for high-medium rectal cancer with curative intent.During the procedure,biopsy of the parietal peritoneum was made before operation and at 45 min,90 min,and 120 min after operation.The tissue-type plasminogen activator (tPA)and plasminogen activator inhibitor-1 (PAI-1 )were determined by enzyme-linked immunosorbent assay in peritoneal tissues.The cellular injury was detected by LDH assay.The proliferation was quantified by MTT assay.Results PAI-1 activity in the peritoneal tissue was significantly lower in LAP group than in the OP group.tPA activity decreased after 45min of open surgery,but there was no significant change in the LAP group.With time extension,the LDH activity increased and the proliferation of the mesothelial cells decreased.Conclusion Preservation of a prolonged hypofibrinolytic state by inhibition of PAI-1 up-regulation during LAP may predispose patients to less postoperative peritoneal adhesion. The cellular injury becomes apparent and the proliferation is inhibited during prolonged laparoscopic surgery.关键词
腹腔镜手术/纤维蛋白溶解作用/组织型纤溶酶原激活物抑制剂/腹膜粘连/间皮细胞Key words
laparoscopy/fibrinolysis/tissue-type plasminogen activator inhibitor-1/peritoneal adhesion/peri-toneal mesothelial cell分类
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赵伟,纪明明,李晓婷,李云东,隋淑凤,谭春林,刘在波,刘莉,王学萍,王康..腹腔镜手术时间延长对腹膜间皮细胞及纤维蛋白溶解活性的影响[J].西安交通大学学报（医学版）,2017,38(1):113-116,4.
