中国组织工程研究2017，Vol.21Issue(2)：182-186,5.DOI:10.3969/j.issn.2095-4344.2017.02.004
骨生物材料复合骨髓间充质干细胞异位成骨修复肋骨大段缺损
Bone biomaterial composited with human bone marrow mesenchymal stem cells for large costal defects
摘要
Abstract
BACKGROUND:Tissue-engineered biomaterials have the similar structure and function with autologous tissues. OBJECTIVE:To explore the osteoinduction of the bone biomaterial composited with rat bone marrow mesenchymal stem cel s in the treatment of large costal defects. METHODS:Forty Wistar rats were enrol ed used for the preparation of right large costal defect models, and then randomized into two groups, fol owed by the implantation of calcium chloride-sodium alginate gel (control group) or chloride-sodium alginate-bone marrow mesenchymal stem cel s (experimental group). At 2, 4 and 8 weeks after implantation, chest X-ray radiograph and histological examination of the defect region were conducted. RESULTS AND CONCLUSION:X-ray showed that in the experimental group, the defect area had no significant changes at the 2nd week after implantation until the formation of few bones at the 4th week;and at the 8th week, both ends of the defect region gradual y connected, and newly formed bones were ful of the defect. In contrast, the defect region in the control group showed no obvious bone healing, and both ends of the defect closed and osteosclerosis occurred. In the experimental group, there were a smal amount of fibrous tissues and numerous inflammatory cel s infiltratied in the material compartment, and no connection occured between the material and broken ends;there were numerous inflammatory cel s but no bone tissues in the control group at the 2nd week. At the 4th week, the scaffold degraded gradual y and abundant bone tissues were seen in the experimental group;the scaffold degraded little, and bone tissues aggregatied at the both defect ends in the control group. Up to the 8th week, the two kinds of scaffolds degraded mostly. A large number of bone tissues and trabeculae formed and the both defect ends were connected with the newly formed bones in the experimental groups, while in the control group, osteosclerosis appeared at both ends of the defect. To conclude, the bone biomaterial composited with rat bone marrow mesenchymal stem cel s promotes the repair of large costal defects.关键词
生物材料/骨生物材料/海藻酸钠/间充质干细胞/组织工程骨/肋骨大段缺损分类
医药卫生引用本文复制引用
王俊钢,李聪聪,毛广显,张洁,杨翠..骨生物材料复合骨髓间充质干细胞异位成骨修复肋骨大段缺损[J].中国组织工程研究,2017,21(2):182-186,5.基金项目
深圳市科技计划项目(JCYJ20140415162338820)；深圳市医疗卫生科研项目(201302068)Funding:the Science and Technology Program of Shenzhen, No. JCYJ20140415162338820 （JCYJ20140415162338820）
the Project of Medical and Health Research of Shenzhen, No.201302068 （）
