中国组织工程研究2017，Vol.21Issue(2)：187-191,5.DOI:10.3969/j.issn.2095-4344.2017.02.005
聚甲基丙烯酸甲酯骨水泥对肺癌细胞毒性作用的体外实验
In vitro toxic effect of polymethylmethacrylate bone cement on lung cancer cells
潘元星 1米川 1施学东 1王冰 1崔云鹏 1林云飞1
作者信息
- 1. 北京大学第一医院骨科，北京市 100083
- 折叠
摘要
Abstract
BACKGROUND:Percutaneous vertebroplasty is a minimal y invasive treatment for spinal metastasis tumor, but the mechanism of the injected polymethylmethacrylate (PMMA) bone cement in the treatment of tumor is not ful y understood. OBJECTIVE:To explore the cytotoxicity of PMMA bone cement and its monomers on tumor cel s. METHODS:PMMA extracts in the wire drawing and curing periods and different mass concentrations of monomer dilutions were co-cultured with human lung cancer cel s spc-A1. The cel morphology was observed using inverted microscope, the absorbance (A) values were detected by cel counting kit-8 assay, the relative growth rate was calculated, and the toxicity of PMAA bone cement and its monomers was evaluated at 1 and 3 days of culture, respectively. RESULTS AND CONCLUSION:At 1 day of culture, the absorbance values in the 1 and 10 g/L groups were significantly lower than that in the negative control group (P<0.01). The absorbance values in the 1 g/L, 10 g/L, 100 mg/L and wire drawing extract groups were significantly lower than those in the negative control group at 3 days of culture (P<0.05). The relative growth rate in the 1 and 10 g/L groups was 26%-29%, and the level of toxicity was grade 4 after 1-day culture;at 3 days, the relative growth rate was decreased to 12%-16%, and the level of toxicity was grades 4-5. After 3-day culture, the level of toxicity reached to grade 2 in the 100 mg/L and wire drawing extract groups. These results indicate that PMMA bone cement in wiredrawing period and its monomers exert toxic effects on tumor cels.关键词
生物材料/骨生物材料/经皮椎体成形术/聚甲基丙烯酸甲酯/骨水泥/细胞毒/肺癌细胞/体外试验/脊柱转移瘤分类
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潘元星,米川,施学东,王冰,崔云鹏,林云飞..聚甲基丙烯酸甲酯骨水泥对肺癌细胞毒性作用的体外实验[J].中国组织工程研究,2017,21(2):187-191,5.
