现代医药卫生2017，Vol.33Issue(3)：340-342,3.DOI:10.3969/j.issn.1009-5519.2017.03.008
联合抗原修复法在免疫组化法检测家兔颈动脉粥样硬化陈旧性蜡块组织MMP-9及TIMP-1表达中的应用
Application of joint antigen retrieval method in detecting MMP-9 and TIMP-1 expressions of rabbit carotid atheroscle-rosis old wax tissue by immunohistochemical staining
摘要
Abstract
Objective To investigate the influence of the joint antigen retrieval method for detecting MMP-9 and TIMP-1 expression of rabbit carotid atherosclerosis old wax tissue by immunohistochemical staining. Methods The serial tissue sections of rabbit carotid atherosclerosis old wax were respectively examined the immunohistochemical staining effects of MMP-9 and TIMP-1 by the pancreatic enzyme,heating and joint antigen retrieval methods. Results The best condition of pancreatic enzyme antigen retrieval method was incubated for 20 min with 0.1%trypsin (pH 7.6) at 37℃. The best condition of heating antigen re-trieval method was 0.01 mol/L EDTA buffer (pH 8.0) for conducting microwave antigen repair. The antigen retrieval method of pancreatic enzymes associated with microwave was superior to the single use of trypsin retrieval or heating retrieval ,the difference was statistically significant (P<0.05). Conclusion The joint antigen retrieval method is more suitable to detect the MMP-9 and TIMP-1 expressions of rabbit carotid atherosclerosis old wax by immunohistochemical staining method.关键词
抗原/免疫组织化学/基质金属蛋白酶-9/组织金属蛋白酶抑制物-1Key words
Antigens/Immunohistochemistry/Matrix metalloproteinase-9/Tissue inhibitor of metalloproteinase-1引用本文复制引用
江齐昌,徐刚,黄跃,陆红玲..联合抗原修复法在免疫组化法检测家兔颈动脉粥样硬化陈旧性蜡块组织MMP-9及TIMP-1表达中的应用[J].现代医药卫生,2017,33(3):340-342,3.基金项目
贵州省科学技术基金资助项目（黔科合J字LKZ[2010]28）；贵州省遵义市汇川区科技局项目（E-121）。 （）
