厦门大学学报（自然科学版）2017，Vol.56Issue(1)：3-12,10.DOI:10.6043/j.issn.0438-0479.201607015
简化基因组测序技术及其在海洋生物研究中的应用
The Reduced-representation Sequencing Technique and Its Applications in Marine Organisms
摘要
Abstract
Traditionally,methods of molecular marker development were expensive and involved iterative,time-consuming PCR process.With the rapid development of next generation sequencing,a very efficient marker development technique,reduced-represen-tation sequencing,has been widely utilized in various research areas.The library construction protocol is simple with low cost.More-over,the reduced-representation sequencing could identify thousands of markers in only one sequencing process.It reduces the com-plexity of the genome,sequences the reduced genome and searches markers on this partial genome.The reduced-representation se-quencing can be grouped into three classes:reduced-representation sequencing,restriction site-associated DNA sequencing (RAD-seq),and low coverage genotyping.This technique has been widely applied in population genetics,phylogenetics,adaptive evolution, linkage map and quantitative trait locus (QTL)mapping studies in marine organisms.关键词
简化基因组测序/海洋生物/基因分型/限制性酶切位点相关DNA测序Key words
reduced-representation sequencing/marine organism/genotyping/restriction site-associated DNA sequencing (RAD-seq)分类
生物科学引用本文复制引用
边力,王军..简化基因组测序技术及其在海洋生物研究中的应用[J].厦门大学学报（自然科学版）,2017,56(1):3-12,10.基金项目
国家自然科学基金(31372504 （）
41476118) （）
