西南林业大学学报2017，Vol.37Issue(1)：1-9,9.DOI:10.11929/j.issn.2095-1914.2017.01.001
基于微卫星分子标记的云南松及其近缘种遗传关系分析
Genetic Relationship of Pinus yunnanensis with Related P ine Species Based on Microsatellite Molecular Markers
摘要
Abstract
Detected the genetic relationship of 7 species or varieties ( forma) belong to the genus Pinus inclu-ding 3 species, 3 varieties and crooked P. yunnanensis, with SSR molecular marking technology in distribution re-gions or neighboring regions of P. yunnanensis. was 100 individuals of 7 species or varieties ( forma) presented 31 alleles with 9 pairs of SSR primers. The number of alleles varied 2 to 6 with an average of 3. 4 for each locus. There were high genetic diversity in P. kesiya var. langbianensis population and low genetic diversity in P. yunnanensis var. tenuifolia. Those species or varieties ( forma) were classified into 3 main groups by UPGMA cluster analysis based on Nei′s (1983) genetic distance. Among them, P. massoniana and P. kesiya var. langbianensis classified as a sep-arate group, respectively, the other 5 species or varieties ( forma) clustered into 1 group. The most similar ones were found between P. yunnanensis and crooked P. yunnanensis and obvious genetic differentiation was not found. The phenotypic degeneration may be caused by the artificial negative selection or environmental degradation.关键词
云南松/SSR/遗传多样性/遗传关系Key words
Pinus yunnanensis/SSR/genetic diversity/genetic relationship分类
农业科技引用本文复制引用
许玉兰,蔡年辉,白青松,何承忠,王大玮,段安安,康向阳..基于微卫星分子标记的云南松及其近缘种遗传关系分析[J].西南林业大学学报,2017,37(1):1-9,9.基金项目
国家自然科学基金项目(31260191、31360189)资助；云南省林学一流学科建设经费资助和云南省高校林木遗传改良与繁育重点实验室开放基金资助；云南省自然科学基金项目(2010CD065)资助。 （31260191、31360189）
