西南林业大学学报2017，Vol.37Issue(1)：10-14,5.DOI:10.11929/j.issn.2095-1914.2017.01.002
贵州南部野生兜兰SRAP遗传多样性分析
Genetic Diversity Analysis of Wild Paphiopedilum Species in the South of Guizhou
摘要
Abstract
SRAP marker was used to detect the genetic diversity of 8 accessions of wild Paphiopedilum species . 8 pairs of polymorphic and repeatable primers were screened out for amplification and establishing the opti-mal PCR reaction system and genetic relationships of wild Paphiopedilum were detected by UPGMA cluster analysis. Totally 152 clear bands were revealed, including 122 polymorphic bands, and the percentage of polymorphic bands was 80. 26%. The mean observed number of alleles ( Na ) was 1. 763 2, the effective number of alleles ( Ne ) was 1. 506 1, the Nei′s gene diversity index ( H) was 0. 287 9 and the Shannon information diversity index ( I) was 0. 424 1. The genetic identity coefficient was between 0. 513 to 0. 756. Divided wild Paphiopedilum from different sources into 3 groups with the threshold value of identity coefficient as 0. 67, the study showed that the clustering of Paphiopedilum species based on the SRAP markers was consistent with that from morphological classification.关键词
兜兰/SRAP/遗传多样性/分子标记Key words
Paphiopedilum/SRAP/genetic diversity/molecular marker分类
农业科技引用本文复制引用
朱亚艳,王港,侯娜,王莲辉..贵州南部野生兜兰SRAP遗传多样性分析[J].西南林业大学学报,2017,37(1):10-14,5.基金项目
贵州省科技厅项目(黔科合SY字[2015]3019号、黔科合NY字[2010]3029号)资助。 （黔科合SY字[2015]3019号、黔科合NY字[2010]3029号）
