西南林业大学学报2017，Vol.37Issue(1)：15-19,35,6.DOI:10.11929/j.issn.2095-1914.2017.01.003
厚壁型和薄壁型巨龙竹的分子标记研究
Study on Thick-Walled and Thin-Walled Types of Dendrocalamus sinicus by Molecular Markers
摘要
Abstract
Study on genetic differentiation of 28 accessions thick-walled and thin-walled Dendrocalcamus sinicus was anaylyzed by using RAPD markers. Out of 88 RAPD primers, 17 primers with good reproducibility and clear ribbon were selected for RAPD markers. 131 loci were identified totally, 118 of which were polymorphic. The ratio of polymorphism was 90. 08%. Polymorphism of thin-walled type was higher than that of thick-walled type. The genetic differentiation coefficient of both types was 0. 154 6. Most of the thin-walled and thick-walled types were re-spectively grouped into one class by cluster analysis ( UPGMA) . It was proved that there were obvious differences between the thick-walled and the thin-walled D. sinicus in DNA.关键词
巨龙竹/厚壁型/薄壁型/RAP D标记/遗传Key words
Dendrocalamus sinicus/thick-walled type/thin-walled type/RAPD marker/heredity分类
农业科技引用本文复制引用
刘世男,辉朝茂,王兵益,刘蔚漪..厚壁型和薄壁型巨龙竹的分子标记研究[J].西南林业大学学报,2017,37(1):15-19,35,6.基金项目
国家自然科学基金项目(31660173)资助；云南省重点新产品开发计划项目(2016BB006)资助；“十三五”国家重点研发计划专题(2016YFD0600902)资助；云南省竹藤科学研究创新团队资助。 （31660173）
