疑难病杂志2017，Vol.16Issue(2)：152-155,4.DOI:10.3969/j.issn.1671-6450.2017.02.012
PD－L1联合CTLA－4检测在胸腔积液患者胸腔积液及外周血单个核细胞中的表达及意义
Expression and significance of PD-L1 and CTLA-4 in pleural effusion and peripheral blood mononuclear cells in pa-tients with pleural effusion
薛珉 1颜志军 1高习文 1庄亚琴 1彭清 1丁静怡1
作者信息
- 1. 201199 上海复旦大学附属闵行医院呼吸内科
- 折叠
摘要
Abstract
Objective To investigate the expression and clinical significance of anti programmed death ligand 1 ( PD-L1) combined with anti cytotoxic T lymphocyte associated antigen ( CTLA-4) in pleural effusion and peripheral blood mononu-clear cells ( PBMC) in patients with pleural effusion .Methods From June 2015 to June 2016 in Shanghai Minhang Hospital Affiliated Fudan University , 84 patients who were diagnosed and treated of pleural effusion were enrolled as the research ob -ject, including 28 cases of tuberculous pleural effusion and 27 cases of malignant pleural effusion , 29 cases of non tuberculous non malignant pleural effusion .Enzyme linked immunosorbent assay ( ELISA ) and polymerase chain reaction ( PCR ) were used to detect the expression of PD-L1, CTLA-4 in PBMC of pleural effusion , and to analyze the diagnostic value of combined detection of two methods for malignant pleural effusion .Results Compared with non tuberculosis non malignant pleural effu-sion, malignant pleural effusion PBMC’s PD-L1, CTLA-4 mRNA, pleural effusion’s PD-L1 and CTLA-4 expression were sig-nificantly increased , while the above indexes in patients with tuberculous pleural effusion was significantly reduced ( P <0.05);the positive correlation between PD-L1 and CTLA-4 in PBMC and pleural effusion ( r =0.477, r =0.418, P <0.05);PD-L1 and CTLA-4 in pleural effusion ’ s joint detection sensitivity and specificity were respectively 92.5% and 94.7%, which were higher than that of pleural effusion exfoliated cells ’ PD-L1, CTLA-4 separate detection, also its sensitivi-ty and specificity was higher than PBMC .Conclusion The expression of PD-L1, CTLA-4 in pleural effusion, PBMC may be related to the etiology , which can reflect the immune function in the local microenvironment .关键词
抗程序性死亡配体-1/抗细胞毒T淋巴细胞相关抗原-4/胸腔积液/外周血单个核细胞Key words
Programmed death ligand 1/Anti cytotoxic T lymphocyte associated antigen/Pleural effusion/Peripheral blood mononuclear cells引用本文复制引用
薛珉,颜志军,高习文,庄亚琴,彭清,丁静怡..PD－L1联合CTLA－4检测在胸腔积液患者胸腔积液及外周血单个核细胞中的表达及意义[J].疑难病杂志,2017,16(2):152-155,4.
