局解手术学杂志2017，Vol.26Issue(1)：9-12,4.DOI:10.11659/jjssx.09E016017
IL-17 A诱导非小细胞肺癌A549细胞分泌CXCL12的机制研究
Mechanism of IL-17A induced the secretion of CXCL12 in non-small cell lung cancer cell line A549
胡英 1黄英 2杨永红1
作者信息
- 1. 重庆市巴南区第二人民医院呼吸科，重庆401320
- 2. 第三军医大学西南医院呼吸科，重庆400038
- 折叠
摘要
Abstract
Objective To study the molecular mechanism of IL-17A induced the secretion of CXCL 12 in non-small cell lung cancer cell line A549.Methods Cultured non-small cell lung cancer cell line A549 in vitro with recombination cytokine IL-17A or STAT3 signal path-way inhibitor pre-incubated for 1 hour, and then the level of CXCL12 were detected by enzyme-linked immunosorbent assay .And chemotaxis assay was used to analyze the chemotactic movement of neutrophil .Results After IL-17A stimulation,the secretion of CXCL12 by non-small cell lung cancer cell line A549 was significantly increased(P<0.01),which is in a dose and time dependent manner .However,IL-17A in-duced the secretion of CXCL12 by A549 was significantly decreased after pre-incubated by the STAT3 inhibitor(P<0.01).In addition,neu-trophil could have chemotaxis by cell suspension obtained from IL-17A stimulated A549 cell line,but such chemotaxis would be declined while CXCL12 was neutralized.Conclusion IL-17A could induce the secretion of CXCL12 in non-small cell lung cancer cell line A549 through STAT3 signal pathway , so as to promote the chemotaxis of neutrophil .关键词
IL-17A/CXCL12/STAT3信号/非小细胞肿瘤/A549细胞Key words
IL-17A/CXCL12/STAT3 signal/non-small cell tumor/A549 cell分类
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胡英,黄英,杨永红..IL-17 A诱导非小细胞肺癌A549细胞分泌CXCL12的机制研究[J].局解手术学杂志,2017,26(1):9-12,4.
