局解手术学杂志2017，Vol.26Issue(1)：13-17,5.DOI:10.11659/jjssx.11E016032
二肽基肽酶抑制剂类似物对LPS诱导的小胶质细胞炎症反应的抑制作用
Inhibitory effect of dipeptide peptidase inhibitors analogues on LPS-induced inflammatory response on microglia
摘要
Abstract
Objective To study the inhibitory effect and mechanism of dipeptide peptidase inhibitors analogues on LPS -induced inflam-matory response on microglia .Methods Microglia cells were cultured ,isolated and purified from the neonatal Sprague-Dawley rats.Divided them into blank group ,negative control ,LPS group and medicine group ( parallel determination for 3 times each group ) after pharmacological preconditioning for 48 hours.The optimal concentration of microglia proliferation induced by LPS were measured by MTT assay .Observed the role of dipeptide peptidase inhibitors analogues on LPS-induced microglia in different concentrations .The interleukin1β( IL-1β) ,tumor necro-sis factor alpha ( TNF-α) were assayed by enzyme-linked immunorrbent assay ( ELISA ) .The expression of TLR-4 was detected by Western blotting and the expression of NF-κB was detected by RT-PCR.Results LPS induced the proliferation of microglia and significantly in-creased the release of inflammatory cytokines in LPS-stimulated primary microglia .Compared with the blank group ,dipeptide peptidase inhibi-tors analogues could inhibit this effect and the IC 50 values was 1.014 ×10 -2 mol/L to MG after pretreatment for 48 hours.Dipeptide peptidase inhibitors analogues could inhibit the release of TNF-αand IL-1 significantly(P<0.01),and it decreased the expression of TLR4 and NF-κB signif-icantly(P<0.01).Conclusion This research suggests that dipeptide peptidase inhibitors analogues restrain cell proliferation and inflammatory re-sponse of LPS-stimulated microglia,and the possible mechanism may be related to the inhibition of the expression of TLR-4 and NF-κB.关键词
二肽基肽酶抑制剂类似物/小胶质细胞/脂多糖/TLR-4/NF-κBKey words
dipeptide peptidase inhibitors analogues/microglia/lipopolysaccharide/TLR-4/NF-κB分类
医药卫生引用本文复制引用
刘颖菊,王松,刘海林,周春巧,彭红梅,文君,陈宇,黄冬梅,范治国..二肽基肽酶抑制剂类似物对LPS诱导的小胶质细胞炎症反应的抑制作用[J].局解手术学杂志,2017,26(1):13-17,5.基金项目
重庆市卫生计生委医学科研基金面上项目 （）
