中国临床医学2016，Vol.23Issue(6)：725-730,6.DOI:10.12025/j.issn.1008-6358.2016.20160709
慢性阻塞性肺疾病患者外周血Th17／CD25＋Foxp3＋Treg比率的变化
Change of Th17/CD25+Foxp3+Treg ratio in peripheral blood of patients with chronic obstructive pulmonary disease and its significance
摘要
Abstract
Objective: To investigate the correlationship between peripheral CD25+ Foxp3+ Treg and Th17 cell‐mediated immune imbalance and the progression of chronic obstructive pulmonary disease (COPD) .Methods:A total of sixty‐nine patients with COPD in stable stage or acute exacerbation of COPD were recruited from Shanghai Fifth People ’s Hospital of Fudan University from January 2012 to March 2015 .At the same time ,the non‐smokers and smokers with normal lung function ,each 20 cases ,were also used as the healthy controls .The pulmonary function of all subjects was evaluated .The percentages of Th17 and CD25+ Foxp3+ Treg cells in CD4+ cells from peripheral blood were determined by flow cytometry . The changes of Th17/CD4+ ,CD25+ Foxp3+ Treg/CD4+ and Th17/CD25+ Foxp3+ Treg ratios in the different groups and their clinical significance were analyzed .Results:Compared with the healthy control group ,the ratios of Th17/CD4+ and Th17/CD25+ Foxp3+ Treg in peripheral blood of patients with stable COPD were significantly increased (P<0 .05) ,while the ratio of CD25+ Foxp3+ Treg/CD4+ was significantly decreased (P<0 .05) .The ratios of Th17/CD4+ ,CD25+ Foxp3+ Treg/CD4+ and Th17/CD25+ Foxp3+ Treg in patients with acute exacerbation of COPD were all increased as compared with the healthy control (all P<0 .05) .With the decline of lung function ,the ratios of Th17/CD4+ and Th17/CD25+ Foxp3+ Treg were gradually up‐regulated in patients with mild ,moderate ,severe ,very severe COPD (P< 0 .05) ,while the ratio of CD25+ Foxp3+ Treg/CD4+ was gradually down‐regulated (P<0 .05) .Moreover ,the ratios of Th17/CD4+ and Th17/CD25+ Foxp3+ Treg in COPD patients were negatively correlated with lung function (P<0 .05) ,while the ratio of CD25+ Foxp3+ Treg/CD4+ was positively correlated with lung function (P< 0 .05) .Conclusions :Th17/CD25+ Foxp3+ Treg imbalance involves in the progression of COPD ,suggesting that Th17/CD25+ Foxp3+ Treg immunomodulatory therapy may become a new approach for the treatment of COPD .关键词
慢性阻塞性肺疾病/免疫调节/T h17细胞/T淋巴细胞Key words
chronic obstructive pulmonary disease/immunomodulation/Th17 cells/T-lymphocytes分类
医药卫生引用本文复制引用
何燕超,揭志军,施劲东,梅周芳,钱凌,黄琦慧,鄢莉宏..慢性阻塞性肺疾病患者外周血Th17／CD25＋Foxp3＋Treg比率的变化[J].中国临床医学,2016,23(6):725-730,6.基金项目
上海市闵行区自然科学基金（2011MHZ03），上海市科学技术委员会自然基金生物引导类项目（134119b1200），上海市卫生系统优秀学科带头人资助项目（13B034），复旦大学青年骨干科研启动基金（11L-34）．Supported by Natural Science Foundation of Minhang District in Shanghai （2011MHZ03），Natural Foundation-Biological Guide Project of Shanghai Municipal Science and Technology Commission （134119b1200），Excellent Academic Leaders of Shanghai Health System （13B034） and Foundation of Start-Up Research by Youth Cadre of Fudan University （11L-34）． （）
