中国临床医学2016，Vol.23Issue(6)：846-850,5.DOI:10.12025/j.issn.1008-6358.2016.20151012
第三代流式细胞分选仪及96孔板分选单个细胞的方法及参数优化
Method and parameter optimization of the third-generation flow cytometry sorter and 96-well plate in single cell sorting
摘要
Abstract
Objective:To compare the sorted single cell rate and cell viability with different diameters of nozzles by the third‐generation flow cytometry sorter FACS Aria Ⅲ so as to further improve the optimization of parameter settings and conditions for single‐cell microplate sorting .Methods :Single‐cell sorting of four different types of cell lines‐Raji cells ,A549 , DT40 ,RAW 264 .7 cell line‐was done on FACSAria Ⅲ flow cytometry sorter and 96‐well microplate .70 μm ,100 μm and 130 μm diameter nozzles were adopted respectively ,and the sorted single cell rate and the cell colony‐forming rate were counted .Results:In the single‐cell sorting mode ,the percentages of sorted single cell with three nozzles were from 86 .55% to 93 .44% ,which meant that the hole numbers with single cell in the 96‐well micorplate were from 84 to 92 .After 7‐day culture , Single cell clone hole numbers sorted by 70 μm , 100 μm and 130 μm diameter nozzles were 31‐52 , 49‐70 and 58‐78 , respectively .Conclusions : In single cell sorting with the microplate and under the premise of accurate adjustment and optimization of parameters ,100 μm or 130 μm nozzles should be given preference for single cell activity .关键词
单细胞分选/流式细胞分选仪/96孔板/喷嘴/液滴延迟Key words
single-cell sorting/flow cytometry sorter/96-well microplate/nozzle/drop delay分类
生物科学引用本文复制引用
闵智慧,程韵枫..第三代流式细胞分选仪及96孔板分选单个细胞的方法及参数优化[J].中国临床医学,2016,23(6):846-850,5.基金项目
国家自然科学基金（81170473）．Supported by National Natural Science Foundation of China （81170473）． （）
