中国免疫学杂志2017，Vol.33Issue(1)：16-19,24,5.DOI:10.3969/j.issn.1000-484X.2017.01.003
原头蚴体外刺激巨噬细胞高表达PPARα/γ并调节其极化
Echinococcus granulosus stimulates high PPARα/γ expression and drives polarization of macrophages in vitro
摘要
Abstract
Objective:To investigate the expression levels of PPARα/γin RAW264. 7 cells in the early stages of co-cultivation with Echinococcus granulosus in vitro. Methods:RAW264. 7 cells were co-cultured with E. granulosus and collected at 12,24,36,48, 72 h. The mRNA levels of PPAR-γ,PPAR-α,M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β,and M2 mac-rophages-associated cytokines including Arg-1,TGF-β and Fizz-1 were detected by qRT-PCR. The protein levels of Arg-1 and MR were analyzed by ELISA. Results:The expression levels of PPAR-γ, PPAR-αand the M2 macrophages-associated cytokines including Arg-1,TGF-β,Fizz-1 and MR were significantly increased,especially at 72 h (P<0. 05). M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β were decreased at 72h although increased at first. Conclusion:During the early stages of co-cultivation with Echinococcus granulosus in vitro, the levels of PPAR-γ/α are up-regulated in RAW264. 7 cells, which may drive macrophage polarization and play a role in the immune escape.关键词
原头蚴/巨噬细胞/PPARα/γ/极化Key words
Echinococcus granulosus/Macrophage/PPAR-γ/α/Polarization分类
医药卫生引用本文复制引用
陈聪哲,董丹,方海瑞,蒋红群,侯隽,杨琨,郭峰,陈雪玲..原头蚴体外刺激巨噬细胞高表达PPARα/γ并调节其极化[J].中国免疫学杂志,2017,33(1):16-19,24,5.基金项目
本文为国家自然科学基金(81360453,81260412)和兵团博士基金(2011BB018)。 （81360453,81260412）
