中国免疫学杂志2017，Vol.33Issue(1)：66-71,6.DOI:10.3969/j.issn.1000-484X.2017.01.013
c-Myc调控PIK3 R3抑制黑色素瘤B16-F10细胞的迁移和侵袭
c-Myc promoted migration and invasion of malignant melanoma B16-F10 cells through regulating PIK3 R3
摘要
Abstract
Objective:To investigate the effect and the related mechanism of c-Myc on the proliferation,invasion and migration ability of malignant melanoma B16-F10 cells. Methods:We detected the expression of PIK3R3 in malignant melanoma and normal tis-sues. Efficiency of gene silencing of c-Myc and PIK3R3 was determined by qPCR and Western blot. We detected the proliferate ability of B16-F10 cells after silencing c-Myc and PIK3R3 using EdU assay. We detected the migration and invasion ability of B16-F10 cells after silencing c-Myc and PIK3R3 using wound healing assays and Transwell matrigel invasion assays. The expression of miR-199a after silencing c-Myc and PIK3R3 using qPCR. The expression of c-Myc and PIK3R3 was detected by qPCR after transfecting miR-199a mimics or miR-199a inhibitor. Dual-luciferase reporter assay system was used to detect miR-199a regulating c-Myc and PIK3R3. Results:Compared normal skin tissue,expression of PIK3R3 was significantly increased in malignant melanoma tissue;after silencing c-Myc and PIK3R3 gene,the proliferation,invasion and metastasis of melanoma cell line B16-F10 were significantly reduced;expression of miR-199a upregulated after silencing c-Myc and PIK3R3 genes, expression of c-Myc and PIK3R3 decreased after transfecting miR-199a mimics, expression of c-Myc and PIK3R3 upregulated after transfecting miR-199a inhibitor, dual luciferase reporter system test results revealed miR-199a can directly regulate c-Myc and PIK3R3 transcription activity. Conclusion: miR199a regulated the expression of c-Myc,then promote proliferation,migration and invasion in malignant melanoma cells.关键词
miR-199a/c-Myc/黑色素瘤/Western blot/Transwell/划痕实验/PIK3R3Key words
miR-199a/c-Myc/Malignant melanoma/Western blot/Transwell/Wound healing assay/PIK3R3分类
医药卫生引用本文复制引用
杨镓宁,邓菲,潘宁..c-Myc调控PIK3 R3抑制黑色素瘤B16-F10细胞的迁移和侵袭[J].中国免疫学杂志,2017,33(1):66-71,6.基金项目
本文为四川省卫计委科研课题(No.140084)。 （No.140084）
