中国全科医学2017，Vol.20Issue(2)：159-164,6.DOI:10.3969/j.issn.1007-9572.2017.02.008
妊娠期糖尿病孕妇大网膜下脂肪组织全基因组 DNA 甲基化研究
Whole Genome DNA Methylation in the Visceral Omental Adipose Tissue of Patients with Gestational Diabetes Mellitus
摘要
Abstract
Background The visceral omental adipose tissues were closely associated with gestational diabetes mellitus (GDM). Till now,there had been few reports about genome - wide methylation profile in visceral omental adipose tissues of GDM. Objective To investigate the pathogenesis of GDM by measuring and comparing the genome - wide DNA methylation patterns in visceral omental adipose tissues between pregnant women with GDM and the normal pregnancies,in order to provide the clues for explaining the mechanism and process of GDM. Methods Pregnant women who accepted regular prenatal examination and hospital delivery in Department of Obstetrics and Gynecology,the First Affiliated Hospital of Kunming Medical University from January 2012 to May 2014 were enrolled. Three cases of GDM were enrolled as GDM group,while three cases of healthy pregnant women as control group. The visceral omental adipose tissues were obtained at time of term caesarean section. The genome - wide DNA of these tissues were extracted. The products were cleaved after degeneration and amplification. The DNA fragment were hybridized with Illumina Methylation BeadChip chip. The methylation level of each gene sites were calculated according to Methylation Analysis Algorithms with deviation correction and infiltration. DAVID database was used to carry out Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway analysis in methylation gene with significant difference. Five cases of pregnant women with and without GDM in the same period were selected to verify the difference of methylation level of candidate gene promoter region. Results 1 298 genes were hypomethylated and 1 570 genes were hypermethylated in GDM group. Among them,the methylation of TMEM195,TCF7L2,IGF1 and IGF1R might be closely associated with regulation of blood glucose level. The methylation of DGKG and NRXN3 might be closely associated with BMI. The GO function analysis supported that the genes with hypomethylation were mainly involved in the biological processes,such as the regulation of cascade reaction activity,the regulation of cell apoptosis and protein transport,the cellular components were mainly extracellular matrix. The biological processes involved in the hypermethylated genes were mainly antigen processing and presentation,and antigen processing and presentation participated by major histocompatibility complex(MHC)Ⅱ. The main cellular components were muscle actin cytoskeleton proteins,MHC and so on. In the analysis of KEGG signal pathway,the pathway of enrichment > 20. 000 had antigen processing and presentation ( 23. 142 ),PPAR ( 22. 068 ). The different methylation gene HLA-G and PPARGC1A were respectively selected from antigen processing and presentation and PPAR signal pathway to validate. The methylation level of HLA-G promoter region in the visceral omental adipose tissues of GDM pregnant women was higher than non GDM pregnant women(t = 4. 968,P = 0. 001). There was no statistically significant difference in the methylation of PPARGC1A promoter region between GDM pregnant women and non GDM pregnant women( t = 0. 929,P= 0. 380). Conclusion There are statistically significant difference in gene methylation sites and levels between GDM and healthy pregnant women. The different methylation gene mainly involve in antigen processing and presentation and PPAR signaling pathway.关键词
糖尿病,妊娠/DNA 甲基化/脂肪组织/抗原呈递/过氧化物酶体增殖物激活受体/全基因组关联研究Key words
Diabetes, gestational/DNA methylation/Adipose tissue/Antigen presentation/Peroxisome proliferator - activated receptors/Genome - wide association study分类
医药卫生引用本文复制引用
钱源,孙浩,肖雪,祁文瑾,张兰,马润玫..妊娠期糖尿病孕妇大网膜下脂肪组织全基因组 DNA 甲基化研究[J].中国全科医学,2017,20(2):159-164,6.基金项目
国家自然科学基金资助项目（81360103）；云南省科技厅（2012FB039）---昆明医学院应用基础研究联合专项资金项目；中央高校基本科研业务费&协和青年基金资助项目 （）
