中国全科医学2017，Vol.20Issue(3)：323-330,8.DOI:10.3969/j.issn.1007-9572.2017.03.012
黄芩素和LY294002对人肝癌细胞系SMMC-7721细胞增殖和凋亡的影响研究
Effects of Baicalein and LY294002 on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell Line SMMC-7721
摘要
Abstract
Objective To study the effects of baicalein and LY294002 on proliferation and apoptosis of human hepatocellular carcinoma cell line SMMC―7721. Methods From March 2015 to January 2016,human hepatocellular carcinoma cell line SMMC―7721 was cultured in vitro to produce cell suspension. The cells were treated by baicalein(1,2,5,10,20, 50,100,200,300 μmol/L,and were respectively named A1,A2,A3,A4,A5,A6,A7,A8,and A9 group),or LY294002(1,2,5,10,20,30 μmol/L,and were respectively named B1,B2,B3,B4,B5,and B6 group). The blank control group and DMSO control group were set up,and the cell proliferation level of SMMC―7721 was measured by CCK8 kit;20 μmol/L baicalein alone(C1 group)or combined with 10 μmol/L LY294002(C2 group)was added to SMMC―7721, the blank control group and DMSO group were set up,and the cell cycle was detected by flow cytometry;2,5,10,20μmol/L baicalein(D1,D2,D3,D4 group were named respectively)was added to SMMC―772,the blank control group and DMSO group were set up,and the cell amount in each group was analyzed by microscopy;20 μmol/L baicalein alone( E1 group)or combined with 10 μmol/L LY294002(E2 group)was added to SMMC―7721,the blank control group and DMSO group were set up,and the early and late apoptosis in each group were measured by flow cytometry;20 μmol/L baicalein alone ( F1 group),10 μmol/L LY294002 alone( F2 group)or combination of these two( F3 group)was added to SMMC ―7721,the blank control group and DMSO group were set up,and the expression level of ERK1/2,CyclinD1,GSK―3β,AKT mRNA was detected by real ― time fluorescence quantification PCR( Real ― time PCR);20 μmol/L baicalein alone( G1 group),10μmol/L LY294002 alone(G2 group)or combination of these two(G3 group)was added to SMMC―7721,the blank control group and DMSO control group were set up,and the expression level of P―ERK1/2,CyclinD1,P-GSK―3β,and P―AKT was analyzed by Western blotting method. Results The cell proliferation level in A8 group,A9 group and B6 group was lower than that in blank control group and DMSO control group(p〈0. 05). The cell ratio at G0/G1 and G2/M period in C2 group was higher than that in blank control group and DMSO group,while the ratio at S period was lower than that in blank control group and DMSO group(p〈0. 05). The cell amount in D4 group was fewer than that in blank control group and DMSO group(p〈0. 05). There was no significant difference in early and late apoptosis among blank control group,DMSO group,E1 group and E2 group(p〉0. 05). The expression level of ERK1/2,CyclinD1,GSK―3β,AKT mRNA in F3 group was lower than that in blank control group and DMSO group(p〈0. 05). The expression level of P―ERK1/2,CyclinD1,P-GSK―3β and P―AKT in G3 group was lower than that in blank control group and DMSO group ( p〈0. 05 ) . Conclusion Baicalein and LY294002 can inhibit the cell proliferation of human hepatocellular carcinoma cell line SMMC ― 7721 , but they cannot influence its apoptosis.关键词
肝肿瘤/细胞增殖/细胞凋亡/黄芩素/LY294002/人肝癌细胞系SMMC-7721Key words
Liver neoplasms/Cell proliferation/Apoptosis/Baicalein/LY294002/Human liver cancer cell line SMMC-7721分类
医药卫生引用本文复制引用
汪枫,喻小兰,夏纪毅,王晓燕,唐利,曹勇,唐小平,夏国栋..黄芩素和LY294002对人肝癌细胞系SMMC-7721细胞增殖和凋亡的影响研究[J].中国全科医学,2017,20(3):323-330,8.基金项目
四川省科技厅-泸州市人民政府-泸州医学院2014年联合科研项目 （）
