中国全科医学2017，Vol.20Issue(6)：678-683,6.DOI:10.3969/j.issn.1007-9572.2017.06.009
哈萨克族原发性高血压病患者全血 DNA 甲基化位点筛查及异常甲基化谱的构建
Blood Screening for DNA Methylation Sites and Construction of Aberrant Methylation Profile in Kazak Essential Hypertensive Patients
摘要
Abstract
Objective To screen aberrant DNA methylation sites of Kazak essential hypertension(EH)by using gene chip technique,and construct Kazak EH aberrant methylation profiles preliminarily,so as to provide a theoretical basis for intensive study of the mechanism of Kazak EH. Methods From June 2014 to October 2015,using random sampling method, 3 042 Kazak herdsmen or semi - nomads were selected as the participants of this study from Urumqi County. Of them,1 487 had EH. And from the 1 487 EH patients,3 were randomly selected as the EH group,and other 3 age - matched healthy volunteers as the control group. Using Illumina Human Methylation 450K BeadChip,we measured the whole blood of all subjects for detecting DNA methylation and screening the aberrant methylation sites of DNA. We analyzed the function of abnormal methylation genes by using Gene Ontology(GO)enrichment analysis and Pathway analysis. Results There were 427 significantly differential methylated sites in EH and normal groups,where in 148 high methylation sites,279 low methylation sites;these sites were located on different chromosomes;among them,chromosome 1 and chromosome 6 were the most,no differential methylated site was found in Y chromosome. GO enrichment analysis showed that aberrant methylation genes mainly involved in the processes of glycoprotein biosynthesis and metabolism,vascular development,vasculature development,collagen binding and so on. Pathway analysis demonstrated that aberrant methylation genes mainly played a part in chondroitin sulfate biosynthesis,insulin signaling pathway,endocytosis and so forth. Conclusion The occurrence of EH involves in the changes in DNA methylation status. A number of differential methylated genes are related with EH associated signaling pathway. This study is only the beginning of construction of Kazak EH genome methylation profile,and the obtained aberrant methylation gene can be considered as a candidate early risk molecular marker of EH. However,more comprehensive and accurate study results need the analysis and verification of a larger sample.关键词
高血压/哈萨克族/DNA 甲基化/基因芯片Key words
Hypertension/Kazak/DNA methylation/Gene chip分类
医药卫生引用本文复制引用
由淑萍,蒋红,刘涛,从美丽,倪国华,詹怀峰,王红军,金晶,马登娥,叶力夏提,赵志强..哈萨克族原发性高血压病患者全血 DNA 甲基化位点筛查及异常甲基化谱的构建[J].中国全科医学,2017,20(6):678-683,6.基金项目
新疆维吾尔自治区自然科学基金资助项目 （）
