中国动物传染病学报Issue(6)：1-5,5.
流感病毒M1蛋白与M2蛋白相互作用以及其在病毒出芽中的作用
INTERACTION BETWEEN M1 AND M2 PROTEINS OF INFLUENZA VIRUS AND ITS ROLE IN VIRUS BUDDING
摘要
Abstract
Influenza virus is an enveloped virus and released from infected cells by budding. M1 protein, a matrix protein of influenza virus, plays an important role in the pathogenicity of the virus and maintenance of the viral morphology. M2 protein, an ion channel protein, plays an important role in virus budding process. Previous studies have shown that M1 protein interacts with M2 protein but the function of this interaction in virus budding remains unclear and the interacting regions of M1 and M2 is unknown. M1 is divided into three regions, N terminal, C end and middle region according to its structure. In the present study, BiFC( biomolecular fluorescence complementation) and VLP(virus-like particle) experiments were conducted to investigate the interaction regions of M1 and M2 and functions of such interactions in virus budding. The results showed that M2 protein interacted with M1 protein through N terminal 1-160 amino acids of M1 protein and M2 protein facilitated budding of M1 protein by interacting with this region of M1 protein. The regions consisting of 1-20 and 140-160 amino acids of M1 protein determined the stability and function of this protein. This study revealed the interacting regions between M1 protein and M2 protein and the function of this interaction in virus budding of influenza virus.关键词
流感病毒/M1蛋白/M2蛋白/出芽Key words
Influenza virus/M1 protein/M2 protein/budding分类
农业科技引用本文复制引用
侯天龙,包旦奇,李泽君,刘芹防,刘焕奇..流感病毒M1蛋白与M2蛋白相互作用以及其在病毒出芽中的作用[J].中国动物传染病学报,2016,(6):1-5,5.基金项目
国家自然科学基金面上项目（31572543）；中央级公益性科研院所基本科研业务费专项资金项目(2016JB13)；中国农业科学院青年英才项目 （2016JB13）
