中国动物传染病学报Issue(6)：12-18,7.
猪伪狂犬病基因缺失灭活疫苗（JS-2012-△gI/gE株）的制备及免疫效力评价
PREPARATION AND IMMUNE EFFICACY EVALUATION OF INACTIVATED SWINE PSEUDORABIES VACCINE (JS-2012-△gI/gE STRAIN)
摘要
Abstract
The Pseudorabies virus (PRV, JS-2012-△gI/gE strain) inactivated with 0.15%formalin was homogenized with MONTANIDE ISA 201 VG (SEPPIC) to prepare the inactivated swine PR vaccine. In order to evaluate the protective efifcacy of the inactivated swine PR vaccine, fifteen 2-week-old piglets which were free of PRV, porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus (PCV2) and classical swine fever virus (CSFV)) were selected randomly and divided equally into three groups. Group 1 were inoculated intramuscularly (i.m.) with 2 mL of inactivated swine Pseudorabies vaccines (JS-2012-△gI/gE strain). At 28 days-post-ifrst inoculation, group 1 and group 2 were inoculated intramuscularly. with the inactivated vaccines at the same dosage. group 3 served as uninoculated control. At 28 days post-inoculation (DPI), all piglets were challenged intranasally with 105.0 TCID50 of PRV (JS-2012 strain). Following the challenge, clinical signs and rectal temperatures were recorded daily for 14 days. The results showed PRV g B antibody levels of piglets vaccinated twice (Group 1) was higher than those vaccinated once (Group 2), The protective efifcacy of the inactivated swine PR vaccine against PRV after double inoculation(Group 1) was better than that after single inoculation (Group 2).关键词
伪狂犬病毒/灭活疫苗/制备/效力评价Key words
Pseudorablies virus/inactivated vaccine/preparation/immune efifcacy evaluation分类
农业科技引用本文复制引用
童武,王涛,郑旭晨,单同领,童光志,李国新,郑浩,刘飞,梁超,李林,田青,曹艳云,武吉强..猪伪狂犬病基因缺失灭活疫苗（JS-2012-△gI/gE株）的制备及免疫效力评价[J].中国动物传染病学报,2016,(6):12-18,7.基金项目
上海市自然科学基金项目（14ZR1448900)；上海市科技兴农重点攻关项目（沪农科攻字（2015）第1-7号）；国家生猪现代产业技术体系项目（CARS-36）；公益性行业（农业）科研专项 （）
