中国动物传染病学报Issue(6)：30-35,6.
典型城市屠宰及销售猪肉中弓形虫的分子检测研究
MOLECULAR DETECTION OF TOXOPLASMAGONDII INFECTION IN SLAUGHTERED AND MARKETING PORK PRODUCTS IN LARGE CITIES
摘要
Abstract
In order to investigate the presence of Toxoplasmagondii infection in pork products in slaughter houses and markets in large cities Chongqing, Guangzhou and Shanghai, 393 pig diaphragmatic muscle samples were collected and treated with pepsin digestion. Genomic DNA of the treated samples were extracted and detected in PCR using a pair of speciifc primers based on a 529 bp repetitive fragment gene of T.gondii. The results showed that the overall positive rate of T.gondii in these 3 cities was 5.34%with the highest in Chongqing (9.83%), followed by Shanghai (3.17%) but no positive samples in Guangzhou. There was no signiifcant difference in positive rates between summer and autumn, or slaughter houses and markets. These results indicated that T. gondii infection occured in pigs in Chongqing and Shanghai. Therefore, comprehensive measures should be implemented to reduce Toxoplasma infection in human and pigs and increase proift of animal industry.关键词
弓形虫/PCR/膈肌/猪Key words
Toxoplasmagondii/PCR/pig/diaphragmatic muscle分类
农业科技引用本文复制引用
詹婷婷,赵权,陈兆国,米荣升,黄燕,陆珂,程龙,贾海燕,胡盼,宋悦,韩先干..典型城市屠宰及销售猪肉中弓形虫的分子检测研究[J].中国动物传染病学报,2016,(6):30-35,6.基金项目
国家自然科学基金项目（31302083）；国家农产品质量安全风险评估重大专项（GJFP201600703）；中央级公益性科研院所基本科研业务费（2016JB13）；闵行区高层次人才科研项目团队资助 （）
