中国现代医学杂志2017，Vol.27Issue(1)：11-15,5.DOI:10.3969/j.issn.1005-8982.2017.01.003
Bax介导硼替佐米诱导胃癌细胞株MKN45凋亡作用的研究*
Bortezomib induced apoptosis of MNK45 cells through up-regulation of Bax
摘要
Abstract
Objective To investigate the effect of Bax on Bortezomib-induced apoptosis of gastric cancer MNK45 cells. Methods Bax shRNA was constructed and transfected into MKN45 cells. Bortezomib of gradient concentrations was administrated into MKN45 cells (5 nmol/L, 25 nmol/L, 50 nmol/L, 100 nmol/L, 250 nmol/L, 500 nmol/L and 1,000 nmol/L). Cell viability and apoptosis rate were examined. Results Bax-knockdown MKN45 cell line was constructed by transfection. Gradient Bortezomib suppressed the proliferation of mock MKN45 cells and induced cell apoptosis, meanwhile Bax protein level was increased. Bortezomib increased apoptosis rate at a concentration of 500 nmol/L. In the shBax group, apoptosis rate was down-regulated and proliferation ability was strengthened. Conclusions Bax-mediated apoptosis caused by Bortezomib and knock-down of Bax could partially attenuate cell death caused by Bortezomib.关键词
胃癌/硼替佐米/BCl- 2相关X/凋亡Key words
gastric cancer/Bortezomib/Bax/apoptosis分类
医药卫生引用本文复制引用
赵羲和,李凯,邹华伟..Bax介导硼替佐米诱导胃癌细胞株MKN45凋亡作用的研究*[J].中国现代医学杂志,2017,27(1):11-15,5.基金项目
国家自然科学基金 （）
