中国现代医学杂志2017，Vol.27Issue(1)：24-28,5.DOI:10.3969/j.issn.1005-8982.2017.01.005
沙门菌去泛素化酶SseL对泛素链的水解特异性研究*
Research on hydrolysis specificity of Salmonella deubiquitinase SseL in hydrolysis of ubiquitin chains
摘要
Abstract
Objective To investigate the specificity of virulence effector protein SseL in hy-drolysis of ubiquitin (Ub) chains. Methods gene was cloned from the serovar Typhi strain Ty2. GST-SseL was expressed and purified and its ability in hydrolysis of Ub-AMC and various ubiquitin chains was studied. Results SseL had only low hydrolytic activity to Ub-AMC and no hydrolytic activity to linear ubiquitin chains. Moreover, SseL showed higher hydrolytic activity to Lys63-linked diubiqui-tin (K63 diUb) than to Lys48-linked diubiquitin (K48 diUb). Conclusions effector protein SseL has higher hydrolytic specificity for isopeptide-linked ubiquitin and higher hydrolytic efficiency to Lys63-linked polyubiquitin.关键词
沙门菌/效应蛋白/去泛素化酶/二泛素/Ub- AMCKey words
Salmonella/effector protein/deubiquitinase/diUb/Ub-AMC分类
医药卫生引用本文复制引用
郑学明,周海森,芮志莲,鞠爱萍,胡红娟,孙溧鸿,王建强,傅行礼..沙门菌去泛素化酶SseL对泛素链的水解特异性研究*[J].中国现代医学杂志,2017,27(1):24-28,5.基金项目
江苏大学科研启动基金项目（No14JDG043）；江苏省重点研发计划（社会发展项目）（NoBE2016663）；江苏省南京市社会发展科技计划项目（No201303032）；江苏省常州市社会发展科技计划-应用基础研究项目 （）
