中国现代医学杂志2017，Vol.27Issue(1)：29-34,6.DOI:10.3969/j.issn.1005-8982.2017.01.006
血必净对爆炸致家兔急性肺损伤的保护作用及其机制研究*
Protective effect of Xuebijing on blast-induced acute lung injury in rabbits and its mechanism
摘要
Abstract
Objective To investigate the effect of Xuebijing on activity of nuclear transcription factor -kB in the lung tissues of rabbits with blast-induced acute lung injury (ALI), and its mechanism. Methods A total of 32 rabbits were randomly divided into 4 groups: normal control group (NS), ALI group, Xuebijing interven-tion groups of different doses (L-XBJ and H-XBJ groups). Each group had 8 rabbits. After the establishment of blast-induced ALI model of rabbits, the rabbits in the L-XBJ and H-XBJ groups were intravenously in-jected with different doses of Xuebijing (10 and 20 ml/kg respectively), while the rabbits in the NS group and the ALI group were injected with normal saline of the same volume. Part of the lung tissues and venous blood were collected at the 24th hour. The wet to dry weight ratio (W/D) was determined. The serum levels of tumor necrosis factor-α (TNF-α) and interleukins (IL-6 and IL-10) were measured by enzyme-linked im-munosorbent assay (ELISA). The expression of NF-κB protein in the lung tissues was evaluated by Western blot. The expression level of NF-κB mRNA in the lung tissues was detected by reverse transcription-poly-merase chain reaction (RT-PCR). The pathological changes of the lung tissues were observed under light mi-croscope. Results The dry to weight ratio in the L-XBJ and H-XBJ groups was lower than that of the ALI group in a concentration-dependent manner. Compared to the ALI group, Xuebijing in the treatment groups greatly inhibited the serum levels of TNF-α and IL-6 and decreased the expression of NF-kB mRNA and protein, but increased the content of IL-10. Pathological examination of the pulmonary tissues indicated severe hemorrhage, edema and abundance of inflammatory cell infiltration in the ALI group; the degree of lung dam-age was obviously reduced in the L-XBJ and H-XBJ groups. Conclusions The interference of Xuebijing could significantly down-regulate the levels of proinflammatory cytokines TNF-α and IL-6, up-regulate anti-inflammatory cytokine IL-10, inhibit the activation of NF-κB; thus block the inflammatory response pathway, alleviate acute lung injury and protect lung tissues.关键词
血必净/急性肺损伤/爆炸伤/核转录因子- κBKey words
Xuebijing/acute lung injury/blast injury/nuclear factor -kB分类
医药卫生引用本文复制引用
黄鑫,郝建,李树雯,马宏昊,李鲁欢,贺慧博,李成恩..血必净对爆炸致家兔急性肺损伤的保护作用及其机制研究*[J].中国现代医学杂志,2017,27(1):29-34,6.基金项目
南京军区医药卫生科研基金 （）
