摘要
Abstract
Objective To investigate the effects of siRNA-induced transient receptor potential channel 7 (TRPM7) on differentiation of human fetal lung fibroblasts to myofibroblasts induced by TGF-β1, providing a new target for the prevention and treatment of pulmonary fibrosis. Methods WI-38 and MRC-5 cells were cultured and stimulated with TGF-β1. The expressions of smooth muscle actin (α-SMA) and collagen Ⅰ were detected by Western blot for evaluation of differentiation of fetal lung fibroblasts to myofibroblasts. The mRNA and protein expressions of TRPM7 in the cells were detected by real-time PCR and Western blot. The cells were divided into control group, model group, nonspecific transfected group and TRPM7 siRNA group. Western blot was performed to detect the expressions of α-SMA, collagen Ⅰ, Smad3, p-Smad3 and Smad7, respectively. Results WI-38 and MRC-5 cells were successfully transformed into muscle fibroblasts in the presence of TGF-β1 (15μg/L) for 24 h. Compared with the control group, the up-regulation of TRPM7, α-SMA, collagen Ⅰ, Smad3 and p-Smad3, and the down-regulation of Smad7 were observed in the model group ( <0.05). Furthermore, the expressions of TRPM7, α-SMA, collagen Ⅰ, Smad3 and p-Smad3 were remarkably down-regulated in the TRPM7 siRNA group compared with the model group ( < 0.05), while TRPM7 siRNA significantly increased Smad7 level compared with the model group ( <0.05). Conclusions TRPM7 siRNA could restrain differentiation of fetal lung fibroblasts to myofibroblasts to some extent, which may be related to the activation of TGF-β1/Smads signal pathway.关键词
瞬时受体电位通道7/肺纤维化/肺成纤维细胞/TGF- β1/Smads信号通路Key words
transient receptor potential channel 7/pulmonary fibrosis/lung fibroblast/TGF-β1/Smads signaling分类
医药卫生
