中国现代医学杂志2017，Vol.27Issue(1)：50-54,5.DOI:10.3969/j.issn.1005-8982.2017.01.010
外周血基因多态性与结直肠癌的相关性及其临床意义*
Correlation of polymorphism of amphiregulin gene in peripheral blood with colorectal cancer and significance
摘要
Abstract
Objective To investigate the relationship of rs2291715 locus polymorphism of amphiregulin ( ) gene in peripheral blood with colorectal cancers and its significance. Methods In this study, 400 colorectal cancer patients treated in our department from Jan. 2012 to Dec. 2015 were recruited as case group, and 400 healthy people who received health examination during the same period were enrolled as con-trol group. Levels of , CEA, CA50 and CA242 in peripheral blood of both groups were separately de-tected. Then PCR amplification combined with DNA direct sequencing was applied to test rs2291715 locus polymorphism of gene in peripheral blood. Based on different genotypes, both groups were classified and observed for the change of level in peripheral blood. Logistic regression analysis was used to eva-luate the relationship between rs2291715 polymorphism of gene and colorectal cancer. Results Serum levels of , CEA, CA50 and CA242 in the case group were higher than those in the control group ( <0.05). There were 2 kinds of allelic genes C and G in the rs2291715 locus of gene, and 3 kinds of genotypes GG, CG and CC were discovered. The frequency of GG and CG genotypes and allelic gene G in the case group was higher than that in the control group ( <0.05). Logistic regression analysis showed that GG genotype and allelic gene G were closely related to the development of colorectal cancer and were the in-dependent risk factors for colorectal cancer [ OR =8.936 (95% CI: 1.539, 48.246), = 0.009]. Conclusions Polymorphism of AREG gene rs2291715 locus is closely related to carcinogenesis and progression of colorectal cancer.关键词
结直肠癌/双向调节蛋白/基因多态性/外周血Key words
colorectal cancer/amphiregulin gene/gene polymorphism/peripheral blood分类
医药卫生引用本文复制引用
吴肖,王爱军,王红钰,徐晋珩,郑宝军,程健,张超,施华,冯俊伟..外周血基因多态性与结直肠癌的相关性及其临床意义*[J].中国现代医学杂志,2017,27(1):50-54,5.基金项目
河北省科技计划项目 （）
