中国现代医学杂志2017，Vol.27Issue(2)：1-12,12.DOI:10.3969/j.issn.1005-8982.2017.02.001
芥子气经腹腔和气管致大鼠急性肺损伤纤维化指标变化*
Changes of pulmonary fibrosis indexes due to sulfur mustard-induced acute lung injury in rats via intraperitoneal and tracheal injection
摘要
Abstract
Objective To establish rat model of sulfur mustard (SM)-induced acute lung injury via intraperitoneal and tracheal injection, in order to compare the differences of pulmonary fibrosis indexes. Methods A total of 136 male Sprague Dawley rats were randomly divided into the five groups, i.e. the control group with 8 rats and other four groups (intraperitoneal SM group, intraperitoneal propylene glycol group, tracheal SM group and tracheal propylene glycol group) with 32 rats in each group. The rats in the intraperitoneal SM group were intraperitoneally injected with 0.1 ml diluted SM (0.96 LD50 = 8 mg/kg), the rats in the tracheal SM group were intratracheally injected with 0.1 ml diluted SM (0.98 LD50=2 mg/kg), the rats in the intraperitoneal propylene glycol group and the tracheal propylene glycol group were injected with 0.1 ml propylene glycol through peritoneal cavity and trachea respectively; meanwhile the status quo was kept with the normal control group. SM-induced pulmonary fibrosis indexes were observed by Masson and immunohistochemical staining. Results Light microscopic observation confirmed that green-stained collagen fibers increased in the alveolar septa at 72nd h. Significantly higher positive expression rates of MMP-2, MMP-9, TIMP-1 and TIMP-2 by immunohistochemical staining in the alveolar septa were detected in the intraperitoneal SM groups compared with those in the tracheal SM groups at each period of time ( < 0.05). The positive expression rates of type Ⅰ collagen and type Ⅲ collagen in the alveolar septa in the intraperitoneal SM groups were significantly increased compared with those in the tracheal SM group at each period of time ( <0.05). Significantly higher positive expression rates of TGF-β1 and Smad7 in the alveolar septa were also observed in the intraperitoneal SM group compared with the tracheal SM group at each period of time ( < 0.05). Conclusions When LD50 concentration of SM through intraperitoneal injection is similar to that through tracheal injection in rat, the indexes of pulmonary fibrosis are significantly increased after intraperitoneal injection compared with those after tracheal injection, suggesting that SM-induced pulmonary fibrosis can be more easily induced through intraperitoneal injection.关键词
芥子气/肺损伤/肺纤维化/大鼠Key words
sulfur mustard/lung injury/pulmonary fibrosis/rat分类
医药卫生引用本文复制引用
韩玮,朱双双,贝媛媛,赵建,钟玉绪,刘菲,赵玉玲,祝筱姬..芥子气经腹腔和气管致大鼠急性肺损伤纤维化指标变化*[J].中国现代医学杂志,2017,27(2):1-12,12.基金项目
国家“重大新药创制”科技重大专项 （）
