中国现代医学杂志2017，Vol.27Issue(2)：13-18,6.DOI:10.3969/j.issn.1005-8982.2017.02.002
线粒体介导产生的活性氧在血管紧张素Ⅱ诱导的肾脏损害中的作用*
Role of mitochondrial reactive oxygen species in angiotensinII-induced renal injury
摘要
Abstract
Objective To clarify the role of mitochondrial reactive oxygen species (ROS) in angiotensin II (AngII)-induced renal injury by use of a specific scavenger of mitochondrial ROS, mitoTEMPO. Methods The mouse model of Ang II-dependent hypertension was induced with infusion of Ang II via subcutaneous miniosmotic pump, and the sham mice were given with normal saline. The Ang II-dependent hypertensive mice were divided into control (Ang II) group and experimental (Ang II + mitoTEMPO) group, which were subcutaneously administered with solvent and mitoTEMPO, respectively. Tail-cuff systolic blood pressure, and urinary excretion of 8-isoprostane and albumin, creatinine clearance, and the levels of mitochondrial ROS in the kidneys were assayed, and pathological changes of renal tissues were analyzed after 4 weeks of treatment. Results Compared with the sham mice, Ang II infusion led to increased systolic blood pressure and urinary excretion of 8-isoprostane and albumin, decreased creatinine clearance, and enhanced glomerulosclerosis index and renal tubulointerstitial injury ( < 0.05). The results were accompanied by the enhanced mitochondrial ROS production in the kidneys ( < 0.05). However, the treatment with mitoTEMPO alleviated all the above changes except for blood pressure, leading to renal protection ( <0.05). Conclusions Treatment with a specific scavenger of mitochondrial ROS, mitoTEMPO, can inhibit renal injury during Ang II-dependent hypertension, which is associated with the decreased mitochondrial ROS production in the kidneys. Thus, the results suggest that mitochondrial ROS can promote Ang II-induced renal injury.关键词
血管紧张素Ⅱ/高血压/肾脏损害/线粒体/活性氧Key words
angiotensin II/hypertension/renal injury/mitochondria/reactive oxygen species分类
医药卫生引用本文复制引用
冯苗苗,刘素晓,王小晓,崔琳,谢世阳,沈思,朱明军,王幼平..线粒体介导产生的活性氧在血管紧张素Ⅱ诱导的肾脏损害中的作用*[J].中国现代医学杂志,2017,27(2):13-18,6.基金项目
国家自然科学基金（No81170243）；河南省科技创新杰出人才项目（No124200510007）；河南省科技攻关计划项目 （）
