中国现代医学杂志2017，Vol.27Issue(2)：19-25,7.DOI:10.3969/j.issn.1005-8982.2017.02.003
MicroRNA-21和microRNA-664减轻肾缺血再灌注损伤的研究*
MicroRNA-21 and microRNA-664 relieve renal ischemia-reperfusion injury
摘要
Abstract
Objective To explore the effect of microRNA-21 and microRNA-664 on renal ischemia-reperfusion injury (IRI). Methods Agomir-21 and agomir-664 were used to pretreat BALB/c mice. The method of bilateral renal pedicle occlusion was adopted to establish the renal IRI model in mice. At the 24th h after renal IRI, the expressions of microRNA-21 and microRNA-664, and their target genes and proteins in the kidneys were detected by real-time fluorescent quantitative PCR and Western blot. The histopathological changes of the kidneys were observed. Results Renal damage was significantly reduced in mice pretreated with agomir-21 and agomir-664, characterized by reduced necrosis of renal tubular epithelial cells. Further study showed that the expressions of microRNA-21 and microRNA-664 and their target genes , and were decreased, the expressions of their target proteins Caspase-3 and ERK1/2 were suppressed. Conclusions MicroRNA-21 and microRNA-664 can reduce renal ischemia-reperfusion injury through regulation of the expressions of their target genes and proteins.关键词
肾缺血再灌注损伤/microRNA- 21/microRNA- 664Key words
renal ischemia-reperfusion injury/microRNA-21/microRNA-664分类
医药卫生引用本文复制引用
胡红林,习小庆,唐伟伟,叶真逢,黄雅为,林双泉,项明峰..MicroRNA-21和microRNA-664减轻肾缺血再灌注损伤的研究*[J].中国现代医学杂志,2017,27(2):19-25,7.基金项目
江西省自然科学基金 （）
