中国现代医学杂志2017，Vol.27Issue(2)：31-35,5.DOI:10.3969/j.issn.1005-8982.2017.02.005
蛋白激酶B（PKB/AKT1）小干扰RNA通过WNT/β-Catenin通路抑制人胃癌细胞株BGC-823的增殖与迁移*
siRNA of PKB/AKT1 inhibits proliferation and migration of BGC-823 cells via WNT/β-catenin signaling pathway
摘要
Abstract
Objective To observe the effect of double-stranded small interfering RNA (siRNA) of protein kinase B (PKB/AKT1) on gastric cancer cell line BGC-823 cell proliferation and migration, and to explore the possible underlying mechanism. Methods BGC-823 cells were cultured and then randomly divided into blank group, negative control group and AKT1 siRNA-transfected group. AKT1 siRNA efficiency was examined through qRT-PCR and Western blot analysis. The proliferation rate of BGC-823 cells was determined through a methyl thiazolyl tetrazolium assay, and the cell migration of BGC-823 cells was examined by scratch assay. The β-catenin, E-Cadherin, N-Cadherin and MMP-9 protein expression levels were determined using Western blot. Results Compared with those of the blank and negative control groups, the expression levels of AKT1 mRNA and protein significantly decreased in the AKT1 siRNA-transfected cells. In addition, the proliferation rate and migration of BGC-823 cells were markedly decreased. The expression level of E-Cadherin increased, whereas β-catenin, N-Cadherin and MMP-9 protein levels were inhibited in the AKT1 siRNA-transfected BGC-823 cells. Conclusions siRNA of AKT1 inhibits BGC-823 cell proliferation and migration via WNT/β-catenin signaling pathway.关键词
蛋白激酶B/胃癌/BGC- 823/WNT/β- CateninKey words
PKB/AKT1/gastric cancer/BGC-823 cell line/WNT/β-catenin分类
医药卫生引用本文复制引用
李树静,张志成,林佳,高超,王忠利..蛋白激酶B（PKB/AKT1）小干扰RNA通过WNT/β-Catenin通路抑制人胃癌细胞株BGC-823的增殖与迁移*[J].中国现代医学杂志,2017,27(2):31-35,5.基金项目
2014年省级大学生创新创业训练计划项目 （）
