中国现代医学杂志2017，Vol.27Issue(2)：36-41,6.DOI:10.3969/j.issn.1005-8982.2017.02.006
mTOR抑制剂对大鼠肝癌生长抑制作用的研究
Inhibition of growth of hepatocellular carcinoma in mice by mTOR inhibitor
蔡靓羽 1张建楠 1尹卫娟 2徐敏逸 2赵劲风2
作者信息
- 1. 南京中医药大学无锡附属医院，江苏 无锡 214071
- 2. 中南大学湘雅医院卫生部纳米生物技术重点实验室，湖南 长沙 410008
- 折叠
摘要
Abstract
Objective To investigate the antitumoral effect of mTOR inhibitor on a HCC model. Methods Hepatoma cells were implanted into livers of syngeneic rats. The rats were treated with the mTOR inhibitor Sirolimus for 4 weeks. Tumor growth was monitored by MR imaging. Antiangiogenic effects were assessed by microvessel density and corrosion casts and by cell proliferation, tube formation and aortic ring assays. Results The Sirolimus-treated rats had significantly longer survival time, smaller tumors, fewer extrahepatic metastases and less ascites than the controls. Sirolimus decreased intratumoral mic rovessel density resulting in extensive tumor necrosis. The Sirolimus concentration for inhibition of endothelial cell proliferation was lower than that for hepatoma cell inhibition. Microtubule formation and vascular sprouting of aortic rings were significantly impaired by mTOR inhibitor. Vascular casts revealed that Sirolimus significantly reduced vascular formation in tumors, but intussusceptive angiogenesis was observed. Conclusions mTOR inhibitor significantly reduces HCC growth and prolongs survival time primarily via antiangiogenic effect. Inhibitors of mTOR may have potential in HCC treatment.关键词
肝癌/西罗莫司/哺乳动物雷帕霉素靶蛋白Key words
hepatocellular carcinoma/Sirolimus/mammalian target of Rapamycin分类
医药卫生引用本文复制引用
蔡靓羽,张建楠,尹卫娟,徐敏逸,赵劲风..mTOR抑制剂对大鼠肝癌生长抑制作用的研究[J].中国现代医学杂志,2017,27(2):36-41,6.
