中国畜牧兽医2017，Vol.44Issue(1)：1-11,11.DOI:10.16431/j.cnki.1671-7236.2017.01.001
东串猪FUT2基因CDS扩增、序列分析及组织表达谱检测
Amplification,Sequence Analysis and Tissue Expression Detection of FUT2 Gene Coding Regions of Dongchuan Pigs
摘要
Abstract
Porcine alpha (1,2)fucosyltransferase (FUT2)gene was importance in glycosphingo-lipid biosynthesis-globo series, potentially played a regulatory role during Escherichia coli (E .coli)F18 infection process in weaned piglets.In order to explore sequence structure of por-cine FUT2 gene and its biological function,this test amplified FUT2 gene CDS sequence of Dong-chuan pigs by PCR,forecasted and analyzed the protein sequences and functional regions of FUT2 gene,its expression level was detected in 11 tissues of 8 Dongchuan weaned piglets in 35 days old at the meantime.The results showed that the CDS sequence of FUT2 gene was 1 023 bp,which encoded 340 amino acids.FUT2 protein was fat-soluble hydrophilic protein,which the structure was not stable,including a transmembrane helix structure,but without signal peptide that sug-gested the FUT2 protein was a membrane protein;FUT2 protein included 2 N-glycosylation sites (No.185 and No.305 amino acids),without O-glycosylation sites,there were 14 potential phos-phorylation sites,included 6 Ser,2 Thr and 6 Tyr,analyzing the functional regions found that the FUT2 protein had a superfamily of conserved domains:FUT1-FUT2-like (58-319 amino acids).The phylogenetic tree result showed that the relative relationship between swine and cat-tle was relatively close,but was distant from chimpanzee,human,mouse and rat.FUT2 gene was expressed in all 11 tissues of Dongchuan weaned piglets,there were higher expression in di-gestive tract and immune tissues.The present results suggested that FUT2 gene might play a role to resistance to E .coli F18 in weaned piglets,and might indirectly against E .coli F18 through the synthesis of fucosyltransferase.关键词
猪/FUT2基因/蛋白结构与功能/表达谱Key words
pig/FUT2 gene/structure and function of protein/expression profile分类
生物科学引用本文复制引用
钦伟云,甘丽娜,赵呈祥,喻礼怀,包文斌,吴圣龙..东串猪FUT2基因CDS扩增、序列分析及组织表达谱检测[J].中国畜牧兽医,2017,44(1):1-11,11.基金项目
国家科技支撑计划（2015BAD03B01）；江苏省科技支撑计划（BE2012330）；苏州市科技支撑计划（SNG201410）；太仓市产学研联合创新资金 （）
