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枯草芽孢杆菌发酵金樱子的工艺优化

武洪志 王芳芳 许灵敏 宋建楼 陈美霞 刁华杰 许丽 刁新平

中国畜牧兽医2017,Vol.44Issue(1):12-21,10.
中国畜牧兽医2017,Vol.44Issue(1):12-21,10.DOI:10.16431/j.cnki.1671-7236.2017.01.002

枯草芽孢杆菌发酵金樱子的工艺优化

Process Optimization of Bacillus subtilis fermented Rose laevigata michx

武洪志 1王芳芳 1许灵敏 1宋建楼 1陈美霞 2刁华杰 1许丽 1刁新平1

作者信息

  • 1. 东北农业大学动物科学技术学院,哈尔滨 150030
  • 2. 高唐六和荣达饲料有限公司,聊城 252800
  • 折叠

摘要

Abstract

The aim of this experiment was to study the optimum process of Rose laevigata michx fermented by Bacillus subtilis .The process of Bacillus subtilis fermented Rose laevigata michx was optimized by test method combining single factor and response surface.The Rosa laevigata michx polysaccharide content was chosen as indexes and the key factors which had greater impact on ferment effect were selected from carbon source,nitrogen source,water content,inoculation amout and fermentation time for further optimize by response surface.The results showed that water content,inoculation amount and fermentation time were the key factors among the five sin-gle factors.When the water content was 53.79%,inoculation amount was 5.74%,fermentation time was 2 days,the average content of Rosa laevigata michx polysaccharide was 15.47% which closed to predicted value (15.36%).Compared with the polysaccharide content in unfermented Rosa laevigata michx ,that of fermented Rosa laevigata michx was increased 47.47%.The fer-mentation process was simple and easy to operate with remarkable fermentation effect,and it could provide theoretical basis for the development of the green feed additive.

关键词

枯草芽孢杆菌/金樱子/饲料添加剂/发酵/工艺优化

Key words

Bacillus subtilis/Rosa laevigata michx/feed additive/fermentation/process optimiza-tion

分类

农业科技

引用本文复制引用

武洪志,王芳芳,许灵敏,宋建楼,陈美霞,刁华杰,许丽,刁新平..枯草芽孢杆菌发酵金樱子的工艺优化[J].中国畜牧兽医,2017,44(1):12-21,10.

基金项目

国家科技支撑计划 ()

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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