中国畜牧兽医2017，Vol.44Issue(1)：22-29,8.DOI:10.16431/j.cnki.1671-7236.2017.01.003
牛leptin成熟肽的克隆及其第3外显子E3-125-C＞A定点突变前后生物信息学对比分析
Cloning of Bovine Leptin Mature Peptide and Bioinformatics Analysis before and after the Third Exon E3-125-C>A Point Mutation
摘要
Abstract
In order to study the differences in the structure of the mature peptide of leptin gene before and after the third exon site directed mutation,and speculated its functional differences, the mature peptide of leptin based on GenBank was amplified using specific primers.And the ma-ture peptide of leptin was cloned and sequenced.The physical and chemical properties,structure and function of encoding products of leptin were compared and analyzed by bioinformatics meth-ods.Leptin single base mutations could change the encoding of amino acids,and result in a sig-nificant difference in the primary structure and secondary structure,and the tertiary structure was relatively stable.Single nucleotide mutations at the E3-125-C > A of leptin gene,caused structural changes that might cause functional changes.关键词
牛/leptin/成熟肽克隆/生物信息学分析/突变Key words
bovine/leptin/cloning of mature peptide/bioinformatics analysis/mutation分类
农业科技引用本文复制引用
李傲楠,师文,张贺,孙倩,王洋..牛leptin成熟肽的克隆及其第3外显子E3-125-C＞A定点突变前后生物信息学对比分析[J].中国畜牧兽医,2017,44(1):22-29,8.基金项目
辽宁省科学技术计划项目 （）
