中国畜牧兽医2017，Vol.44Issue(1)：30-37,8.DOI:10.16431/j.cnki.1671-7236.2017.01.004
水貂白细胞介素-4基因的克隆、序列分析与原核表达
Cloning,Sequence Analysis and Prokaryotic Expression of Mink Interleukin-4 Gene
摘要
Abstract
In order to obtain high purity of interleukin 4 (IL-4)protein,the specific primers were designed and synthesized according to the mink IL-4 gene sequence in GenBank.The IL-4 gene was amplified by RT-PCR using total RNA of the lymphocyte isolated from the peripheral blood that induced by phytohemagglutinin (PHA).The length of IL-4 gene sequence was 399 bp which encoded 132 amino acids.Phylogenetic analysis revealed that the amino acid sequences homology between mink and ferret was 99.2%,90.0% with Ailuropoda melanoleuca and Canis familiaris . Prokaryotic expression vector pProEX-HTb-IL-4 was constructed and induced by IPTG.The re-combinant protein of 15 ku was isolated by SDS-PAGE and detected by Western blotting.Highly purified recombinant protein of mink IL-4 was obtained by His-Trap HP affinity columns meth-od.This research laid the foundation for the further studies on the biological function of mink IL-4 gene.关键词
水貂/白介素-4/序列分析/原核表达Key words
mink/interleukin-4/sequence analysis/prokaryotic expression分类
生物科学引用本文复制引用
范思宁,赵辉,廉士珍,闫喜军,张海玲,赵建军,王洋,胡博,吕爽,马凡舒,李欣彤,凌明玉..水貂白细胞介素-4基因的克隆、序列分析与原核表达[J].中国畜牧兽医,2017,44(1):30-37,8.基金项目
吉林省科技发展计划项目（20140520172JH）；吉林省科技发展计划项目 （）
