中国畜牧兽医2017，Vol.44Issue(1)：44-52,9.DOI:10.16431/j.cnki.1671-7236.2017.01.006
新型多肽表位技术研究进展
Research Progress on Novel Peptide Epitopes Technology
摘要
Abstract
As an important component of the antigen,peptide epitopes can be recognized by T cell receptor (TCR)and antibodies which induced protective immunity by the host immune system. Traditional research methods,such as overlapping peptide synthesis is long time-consuming which greatly limits the study of epitopes. With the development of bioinformatics,high-throughput sequencing,CRISPR/Cas9 technology,MS and other technologies and explaining the processing mechanism of TAP-dificient protein constantly,the research for epitopes is being per-fect continually.In this article,some new methods to study epitopes were introduced,including computer prediction of B cell or T cell epitopes,identification of epitopes based on the structure and interaction of MHC and antigenic peptides,screening epitopes by means of high throughput sequencing,screening epitopes by means of newly genome editing,the up-to-date research in TAP-deficient T cell epitopes,etc.Finally,future prospect for research direction of peptides was further forecasted.关键词
多肽/表位/筛选/CRISPR/Cas9技术/TAP非依赖性表位Key words
peptide/epitope/screening/CRISPR/Cas9 technology/TAP-dificient epitopes分类
农业科技引用本文复制引用
韩勇,高花,翟晓鑫,高凤山..新型多肽表位技术研究进展[J].中国畜牧兽医,2017,44(1):44-52,9.基金项目
国家自然科学基金项目猪源病毒 CTL 多肽表位与 SLA-Ⅰ结晶研究 （）
