中国畜牧兽医2017，Vol.44Issue(1)：74-79,6.DOI:10.16431/j.cnki.1671-7236.2017.01.010
移植骨髓间充质干细胞对萨能奶山羊乳腺组织发育及血清激素水平的影响
Effect of Bone Marrow Mesenchymal Stem Cells Transplantation on the Mammary Tissue Development and Serum Hormone Level of Saanen Dairy Goat
摘要
Abstract
The study was aimed to analyze the effect of transplanted bone marrow mesenchymal stem cells (BMSCs)on the mammary gland development and serum hormone level of Saanen dairy goat.16 healthy goats with 2 months old and similar body weight were randomly divided in-to control group and experimental group.The BMSCs which was amplified to the P6 generation was injected to the goat via jugular vein,and the control group was injected with the same amount of normal saline.Feeding and management of the two groups were the same.Blood samples were collected monthly,and mammary gland tissue samples were collected at 3,5 and 7 months old. Frozen sections of mammary gland tissue was prepared,the ducts and acinus numbers were ob-served and recorded.The results showed that after transplantation of BMSCs,at 5 and 7 months old,the ducts and acinus number of goat in experimental group were significantly higher than that of control group (P <0.05).At 5,7,8 and 9 months old,the serum E2 level of goat in experimen-tal group was significantly higher than that of control group (P <0.05);At 5 to 9 months old,the serum P level in experimental group was significantly higher than that of control group (P <0.05).At 7 to 9 months old,the serum PRL and INS levels in experimental group were signifi-cantly higher than that of the control group (P <0.05);At 8 and 9 months old,the serum levels of GnRH level in experimental group was significantly higher than that of the control group (P <0.05).The serum LEP,CRH and ACTH levels were higher than that of the control group at the whole test process,but the difference was not significant (P >0.05).In conclusion,injection of BMSCs could increase the duct and acinus number of dairy goat mammary,improve the hormone level in serum,and promote mammary gland development.关键词
骨髓间充质干细胞/导管/腺泡/激素/乳腺发育Key words
BMSCs/duct/acinus/hormone/mammary gland development分类
农业科技引用本文复制引用
邵伟,李杨,王立文,李翠,木合依扎·热很别克,余雄..移植骨髓间充质干细胞对萨能奶山羊乳腺组织发育及血清激素水平的影响[J].中国畜牧兽医,2017,44(1):74-79,6.基金项目
国家自然基金（31560645）；新疆维吾尔自治区重点实验室开放课题（2015KL018）；中国博士后基金委资助 （）
