中国畜牧兽医2017，Vol.44Issue(1)：113-122,10.DOI:10.16431/j.cnki.1671-7236.2017.01.016
鸡蛔虫线粒体cox1和nad4基因的遗传变异分析
Genetic Variation Analysis of Mitochondrial cox1 and nad4 Genes of Ascaridia galli
摘要
Abstract
To study the genetic variations of Ascaridia galli (A.galli)and the phylogentic rela-tionships with other Ascaridata,fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1)gene and complete sequence of NADH dehydrogenase subunit 4 (nad4)gene of 15 adult A.galli isolated from Xichang city in Sichuan province were amplified by PCR,then analyzed the sequences.NJ trees and Bayes trees based on pcox1 and pnad4 genes were reconstructed.The partial sequences of cox1 gene and complete sequence of nad4 gene were 1 152 and 1 236 bp, respectively.There were 33 and 40 variable sites in the pcox1 and nad4 gene sequences,the in-traspecific sequence variations within A.galli were 0 to 2.1% for pcox1 gene,0 to 2.3% for nad4 gene.8 and 11 haplotypes were detected in pcox1 and nad4 genes,the global haplotype diversity were 0.733 ± 0.124 and 0.933 ± 0.054,the nucleotide diversities were 0.00433 ± 0.00153 and 0.00541±0.00157,and the average genetic distances were 0.004 and 0.005,respec-tively.Phylogenetic trees based on pcox1 and pnad4 genes with Neighbour-Joining and Bayesian analysis method revealed that all A.galli were clustered in one clade,and they were more closely related to A.columbae than any other members of Ascaridata.These findings indicated that 15 isolates of A.galli from Xichang city kept low genetic variation,nad4 gene was more suitable and effective than cox1 gene as molecular marker for detecting genetic variation of A.galli.关键词
鸡蛔虫/cox1基因/nad4基因/遗传变异Key words
Ascaridia galli/cox1 gene/nad4 gene/genetic variation分类
农业科技引用本文复制引用
郝桂英,何学谦,万润..鸡蛔虫线粒体cox1和nad4基因的遗传变异分析[J].中国畜牧兽医,2017,44(1):113-122,10.基金项目
四川省科技厅重点项目（2014NZ0113）；攀西动物疫病检测与防控四川省高校重点实验室基本科研业务费项目 （）
