中国畜牧兽医2017，Vol.44Issue(1)：155-160,6.DOI:10.16431/j.cnki.1671-7236.2017.01.021
糖对牦牛卵母细胞体外成熟及其发育能力的影响
Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes
摘要
Abstract
This study was designed to investigate the effect of different types and different concen-trations of sugar on in vitro maturation(IVM)and developmental competence of yak oocytes,for being further research and optimization culture system of yak oocytes for efficient maturity yak oocytes and productivity of embryos.Immature yak oocytes were matured in vitro on culture me-dium with different concentrations (0,5 and 10 mmol/L)of glucose and sucrose in incubator for 24 h or 2 h pretreament with sugar and 22 h without sugar.Subsequently,then the maturation of oocytes,the cleavage rates and blastocyst formation rates after in vitro fertilization(IVF)were evaluated.The results showed that a medium with 5 and 10 mmol/L glucose IVM could signifi-cantly increase the yak oocytes maturation and cleavage (P <0.05),and the highest blastocyst formation rates in 10 mmol/L glucose group was significantly higher than 0 mmol/L glucose (P <0.05).10 mmol/L sucrose could increase significantly the nucleus maturation rates (P <0.05), and there was no significant difference of the blastocyst formation rates after IVF between 0 and 10 mmol/L sucrose (P >0.05).Furthermore,the nucleus maturation rates,IVF cleavage rates and blastocyst formation rates of yak oocytes which pretreated with 10 mmol/L glucose were the highest in these groups,and were higher than 0 mmol/L glucose (P <0.05).It manifested that the appropriate concentration of sugar could improve the quality of yak oocytes and embryos in vitro developmental competence,so it influenced in vitro development of yak oocytes indirectly.关键词
卵母细胞/糖/体外成熟/牦牛Key words
oocyte/sugar/in vitro maturation/yak分类
农业科技引用本文复制引用
石仙,熊显荣,兰道亮,胡嘉嘉,蔡雯,张大伟,李键..糖对牦牛卵母细胞体外成熟及其发育能力的影响[J].中国畜牧兽医,2017,44(1):155-160,6.基金项目
四川省科技支撑计划（2014NZ0114）；西南民族大学研究生创新型科研项目 （）
