中国畜牧兽医2017，Vol.44Issue(1)：186-193,8.DOI:10.16431/j.cnki.1671-7236.2017.01.026
猪嗜淋巴细胞疱疹病毒2型GD27株gB基因生物信息学分析
Bioinformatics Analysis of gB Gene of Porcine Lymphotropic Herpesviruses Strain GD27
摘要
Abstract
The glycoproteins B (gB)gene of porcine lymphotropic herpesviruses 2 (PLHV-2)was amplified by PCR and sequenced,then bioinformatics analysis were conducted.The gB protein was composed of 876 amino acids,the molecular formula was C4500 H6975 N1199 O1351 S40 ,the molecu-lar mass was 100.77 ku,the value of theoretical isoelectric point was 6.45,and the instability in-dex was 38.58.The prediction of secondary structure revealed that some alpha helixes and beta sheets exist in protein while random coil was major pattern.It had a signal peptide in the position 1-39 amino acids and a transmembrane helice in the position 761-780 amino acids.It contained several prosites and several intrinsically disordered proteins.Tertiary structure model of gB pro-tein showed a fusion loop.Multiple antigenic epitope located in gB through comprehensive analy-sis prediction method.It was suggested that gB gene could be as a candidate antigen for vaccina-tion of PLHV-2.关键词
猪嗜淋巴细胞疱疹病毒/gB基因/生物信息学分析Key words
porcine lymphotropic herpesviruses/gB gene/bioinformatics analysis分类
农业科技引用本文复制引用
朱事康,佟铁铸,刘星,李春萍,陈燕忠,罗卓军,林志雄,刘中勇,周宇..猪嗜淋巴细胞疱疹病毒2型GD27株gB基因生物信息学分析[J].中国畜牧兽医,2017,44(1):186-193,8.基金项目
广东省科技厅项目 （）
